Retinoid-regulated expression of BCL-2 and tissue transglutaminase during the differentiation and apoptosis of human myeloid leukemia (HL-60) cells

L. Nagy, Vilmos A. Thomázy, Roshantha A S Chandraratna, Richard A. Heyman, Peter J A Davies

Research output: Contribution to journalArticle

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Abstract

Retinoids induce terminal differentiation and subsequent apoptosis in the human myeloid leukemia (HL-60) cell line. We have previously shown that in HL-60 cells, ligand activation of retinoic acid receptors (RARs) is sufficient to induce differentiation but ligand activation of retinoid X receptors (RXRs) is necessary for the retinoid-induced apoptosis of these cells. In the present studies we have characterized the effect of retinoids on the expression of two apoptosis-linked gene products, BCL-2 and tissue transglutaminase. BCL-2 is a membrane-associated protein whose expression has been linked to the suppression of apoptosis in many cells. Tissue transglutaminase is a protein cross-linking enzyme that accumulates in many cells undergoing apoptotic cell death. Our data suggest that ligand activation of RARs in HL-60 cells results in a global suppression of BCL-2 expression whereas ligand activation of both RARs and RXRs triggers the selective accumulation of tissue transglutaminase in the apoptotic HL-60 cells.

Original languageEnglish
Pages (from-to)499-505
Number of pages7
JournalLeukemia Research
Volume20
Issue number6
DOIs
Publication statusPublished - Jun 1996

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Myeloid Leukemia
HL-60 Cells
Retinoids
Retinoic Acid Receptors
Apoptosis
Ligands
Retinoid X Receptors
Membrane Proteins
Cell Death
Cell Line
transglutaminase 2
Enzymes
Genes
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Retinoid-regulated expression of BCL-2 and tissue transglutaminase during the differentiation and apoptosis of human myeloid leukemia (HL-60) cells. / Nagy, L.; Thomázy, Vilmos A.; Chandraratna, Roshantha A S; Heyman, Richard A.; Davies, Peter J A.

In: Leukemia Research, Vol. 20, No. 6, 06.1996, p. 499-505.

Research output: Contribution to journalArticle

Nagy, L. ; Thomázy, Vilmos A. ; Chandraratna, Roshantha A S ; Heyman, Richard A. ; Davies, Peter J A. / Retinoid-regulated expression of BCL-2 and tissue transglutaminase during the differentiation and apoptosis of human myeloid leukemia (HL-60) cells. In: Leukemia Research. 1996 ; Vol. 20, No. 6. pp. 499-505.
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