Retinoic acid enhances histamine content and H1 receptor expression in human neuroblastoma cell line Paju

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Abstract

Background: A human neuroblastoma cell line (Paju) was induced by retinoic acid (RA) to differentiate into neuron-like cells. Materials and Methods: We studied the expression and the possible role of histamine receptors H1 and H2 in retinoic-acid mediated differentiation by semiquantitative RT-PCR. We studied the effect of exogeneously added RA on the morphological change of the human neuroblastoma cell line and the differentiation was followed by vimentine, glial fibrillary acidic protein (GFAP) and neurofilament (NF) immunostaining. We monitored the change of the histidine decarboxylase (HDC) expression and the histamine content during the RA treatment by immunoblot and flow cytometry methods. Results: Our data showed that H1 and H2 histamine receptors are present on Paju cells. Ten nM RA markedly increased the H1 receptor expression of these cells, while the H2 expression was unchanged. Conclusion: In the RA-treated Paju cells, the histamine content increased compared to the untreated cells, suggesting that neuroblastoma-derived histamine is involved in the regulation of RA-induced in vitro differentiation by H1 receptors.

Original languageEnglish
Pages (from-to)1657-1663
Number of pages7
JournalAnticancer Research
Volume24
Issue number3 A
Publication statusPublished - May 2004

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Histamine H1 Receptors
Tretinoin
Neuroblastoma
Cell Line
Histamine
Histamine H2 Receptors
Histidine Decarboxylase
Intermediate Filaments
Glial Fibrillary Acidic Protein
Cell Differentiation
Flow Cytometry
Neurons
Polymerase Chain Reaction

Keywords

  • Histamine
  • Histamine receptors
  • Neuroblastoma
  • Retinoic acid
  • Tumor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

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title = "Retinoic acid enhances histamine content and H1 receptor expression in human neuroblastoma cell line Paju",
abstract = "Background: A human neuroblastoma cell line (Paju) was induced by retinoic acid (RA) to differentiate into neuron-like cells. Materials and Methods: We studied the expression and the possible role of histamine receptors H1 and H2 in retinoic-acid mediated differentiation by semiquantitative RT-PCR. We studied the effect of exogeneously added RA on the morphological change of the human neuroblastoma cell line and the differentiation was followed by vimentine, glial fibrillary acidic protein (GFAP) and neurofilament (NF) immunostaining. We monitored the change of the histidine decarboxylase (HDC) expression and the histamine content during the RA treatment by immunoblot and flow cytometry methods. Results: Our data showed that H1 and H2 histamine receptors are present on Paju cells. Ten nM RA markedly increased the H1 receptor expression of these cells, while the H2 expression was unchanged. Conclusion: In the RA-treated Paju cells, the histamine content increased compared to the untreated cells, suggesting that neuroblastoma-derived histamine is involved in the regulation of RA-induced in vitro differentiation by H1 receptors.",
keywords = "Histamine, Histamine receptors, Neuroblastoma, Retinoic acid, Tumor",
author = "H. Hegyesi and Z. Darvas and V. L{\'a}szl{\'o} and Z. P{\'o}s and E. P{\'a}llinger and K. Hirschberg and P. Kov{\'a}cs and A. Falus",
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TY - JOUR

T1 - Retinoic acid enhances histamine content and H1 receptor expression in human neuroblastoma cell line Paju

AU - Hegyesi, H.

AU - Darvas, Z.

AU - László, V.

AU - Pós, Z.

AU - Pállinger, E.

AU - Hirschberg, K.

AU - Kovács, P.

AU - Falus, A.

PY - 2004/5

Y1 - 2004/5

N2 - Background: A human neuroblastoma cell line (Paju) was induced by retinoic acid (RA) to differentiate into neuron-like cells. Materials and Methods: We studied the expression and the possible role of histamine receptors H1 and H2 in retinoic-acid mediated differentiation by semiquantitative RT-PCR. We studied the effect of exogeneously added RA on the morphological change of the human neuroblastoma cell line and the differentiation was followed by vimentine, glial fibrillary acidic protein (GFAP) and neurofilament (NF) immunostaining. We monitored the change of the histidine decarboxylase (HDC) expression and the histamine content during the RA treatment by immunoblot and flow cytometry methods. Results: Our data showed that H1 and H2 histamine receptors are present on Paju cells. Ten nM RA markedly increased the H1 receptor expression of these cells, while the H2 expression was unchanged. Conclusion: In the RA-treated Paju cells, the histamine content increased compared to the untreated cells, suggesting that neuroblastoma-derived histamine is involved in the regulation of RA-induced in vitro differentiation by H1 receptors.

AB - Background: A human neuroblastoma cell line (Paju) was induced by retinoic acid (RA) to differentiate into neuron-like cells. Materials and Methods: We studied the expression and the possible role of histamine receptors H1 and H2 in retinoic-acid mediated differentiation by semiquantitative RT-PCR. We studied the effect of exogeneously added RA on the morphological change of the human neuroblastoma cell line and the differentiation was followed by vimentine, glial fibrillary acidic protein (GFAP) and neurofilament (NF) immunostaining. We monitored the change of the histidine decarboxylase (HDC) expression and the histamine content during the RA treatment by immunoblot and flow cytometry methods. Results: Our data showed that H1 and H2 histamine receptors are present on Paju cells. Ten nM RA markedly increased the H1 receptor expression of these cells, while the H2 expression was unchanged. Conclusion: In the RA-treated Paju cells, the histamine content increased compared to the untreated cells, suggesting that neuroblastoma-derived histamine is involved in the regulation of RA-induced in vitro differentiation by H1 receptors.

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KW - Histamine receptors

KW - Neuroblastoma

KW - Retinoic acid

KW - Tumor

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