Retention/quantitation properties of the o-phthaldialdehyde-3-mercaptopropionic acid and the o-phthaldialdehyde-N-acetyl-L-cysteine amino acid derivatives in reversed-phase high-performance liquid chromatography

A. Vasanits, D. Kutlán, P. Sass, I. Molnár-Perl

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The separation/identification of 25 amino acids as their o-phthaldialdehyde-3-mercaptopropionic acid (OPA/MPA) and o-phthaldialdehyde-N-acetyl-L-cysteine (OPA/NAC) derivatives have been optimized [paying particular attention to those amino acids which elute with more than one derivative (glycine, histidine, γ-aminobutyric acid, β-alanine, ornithine, lysine) and that are expected to be present in apples in their free form]. Optimum separation conditions are reported on six reversed-phase columns: Nucleosil 3 and 5 μm, 150(+20 guard)x4.0 mm; Gromsil 3 μm, 150(+10 guard)x4.0 mm; Hypersil 5 μm, 150(+20 guard)x4.0 mm and 200(+20 guard)x4.0 mm; and Hypersil 3 μm, 150(+20 guard)x4.0 mm. Elutions were followed, simultaneously, with photodiode array and fluorescence detectors connected in line. Optimization studies carried out in model solutions as a function of temperature (30-55°C) and eluent flow-rate (0.8-2.5 mL/min) demonstrated that optimum resolutions are obtained with the highest flow-rate applicable (remaining on the safe side with a column pressure of

Original languageEnglish
Pages (from-to)271-287
Number of pages17
JournalJournal of Chromatography A
Volume870
Issue number1-2
DOIs
Publication statusPublished - Feb 18 2000

Fingerprint

3-Mercaptopropionic Acid
o-Phthalaldehyde
Acetylcysteine
High performance liquid chromatography
Reverse-Phase Chromatography
High Pressure Liquid Chromatography
Flow rate
Derivatives
Aminobutyrates
Amino Acids
Ornithine
Malus
Photodiodes
Histidine
Alanine
Glycine
Lysine
Fluorescence
Detectors
Pressure

Keywords

  • Amino acids
  • Apples
  • Derivatization, LC
  • Food analysis
  • Fruits

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

@article{8dbc9d17e0fb4aee90d10654626360eb,
title = "Retention/quantitation properties of the o-phthaldialdehyde-3-mercaptopropionic acid and the o-phthaldialdehyde-N-acetyl-L-cysteine amino acid derivatives in reversed-phase high-performance liquid chromatography",
abstract = "The separation/identification of 25 amino acids as their o-phthaldialdehyde-3-mercaptopropionic acid (OPA/MPA) and o-phthaldialdehyde-N-acetyl-L-cysteine (OPA/NAC) derivatives have been optimized [paying particular attention to those amino acids which elute with more than one derivative (glycine, histidine, γ-aminobutyric acid, β-alanine, ornithine, lysine) and that are expected to be present in apples in their free form]. Optimum separation conditions are reported on six reversed-phase columns: Nucleosil 3 and 5 μm, 150(+20 guard)x4.0 mm; Gromsil 3 μm, 150(+10 guard)x4.0 mm; Hypersil 5 μm, 150(+20 guard)x4.0 mm and 200(+20 guard)x4.0 mm; and Hypersil 3 μm, 150(+20 guard)x4.0 mm. Elutions were followed, simultaneously, with photodiode array and fluorescence detectors connected in line. Optimization studies carried out in model solutions as a function of temperature (30-55°C) and eluent flow-rate (0.8-2.5 mL/min) demonstrated that optimum resolutions are obtained with the highest flow-rate applicable (remaining on the safe side with a column pressure of",
keywords = "Amino acids, Apples, Derivatization, LC, Food analysis, Fruits",
author = "A. Vasanits and D. Kutl{\'a}n and P. Sass and I. Moln{\'a}r-Perl",
year = "2000",
month = "2",
day = "18",
doi = "10.1016/S0021-9673(99)00942-5",
language = "English",
volume = "870",
pages = "271--287",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Retention/quantitation properties of the o-phthaldialdehyde-3-mercaptopropionic acid and the o-phthaldialdehyde-N-acetyl-L-cysteine amino acid derivatives in reversed-phase high-performance liquid chromatography

AU - Vasanits, A.

AU - Kutlán, D.

AU - Sass, P.

AU - Molnár-Perl, I.

PY - 2000/2/18

Y1 - 2000/2/18

N2 - The separation/identification of 25 amino acids as their o-phthaldialdehyde-3-mercaptopropionic acid (OPA/MPA) and o-phthaldialdehyde-N-acetyl-L-cysteine (OPA/NAC) derivatives have been optimized [paying particular attention to those amino acids which elute with more than one derivative (glycine, histidine, γ-aminobutyric acid, β-alanine, ornithine, lysine) and that are expected to be present in apples in their free form]. Optimum separation conditions are reported on six reversed-phase columns: Nucleosil 3 and 5 μm, 150(+20 guard)x4.0 mm; Gromsil 3 μm, 150(+10 guard)x4.0 mm; Hypersil 5 μm, 150(+20 guard)x4.0 mm and 200(+20 guard)x4.0 mm; and Hypersil 3 μm, 150(+20 guard)x4.0 mm. Elutions were followed, simultaneously, with photodiode array and fluorescence detectors connected in line. Optimization studies carried out in model solutions as a function of temperature (30-55°C) and eluent flow-rate (0.8-2.5 mL/min) demonstrated that optimum resolutions are obtained with the highest flow-rate applicable (remaining on the safe side with a column pressure of

AB - The separation/identification of 25 amino acids as their o-phthaldialdehyde-3-mercaptopropionic acid (OPA/MPA) and o-phthaldialdehyde-N-acetyl-L-cysteine (OPA/NAC) derivatives have been optimized [paying particular attention to those amino acids which elute with more than one derivative (glycine, histidine, γ-aminobutyric acid, β-alanine, ornithine, lysine) and that are expected to be present in apples in their free form]. Optimum separation conditions are reported on six reversed-phase columns: Nucleosil 3 and 5 μm, 150(+20 guard)x4.0 mm; Gromsil 3 μm, 150(+10 guard)x4.0 mm; Hypersil 5 μm, 150(+20 guard)x4.0 mm and 200(+20 guard)x4.0 mm; and Hypersil 3 μm, 150(+20 guard)x4.0 mm. Elutions were followed, simultaneously, with photodiode array and fluorescence detectors connected in line. Optimization studies carried out in model solutions as a function of temperature (30-55°C) and eluent flow-rate (0.8-2.5 mL/min) demonstrated that optimum resolutions are obtained with the highest flow-rate applicable (remaining on the safe side with a column pressure of

KW - Amino acids

KW - Apples

KW - Derivatization, LC

KW - Food analysis

KW - Fruits

UR - http://www.scopus.com/inward/record.url?scp=0033985577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033985577&partnerID=8YFLogxK

U2 - 10.1016/S0021-9673(99)00942-5

DO - 10.1016/S0021-9673(99)00942-5

M3 - Article

C2 - 10722085

AN - SCOPUS:0033985577

VL - 870

SP - 271

EP - 287

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

IS - 1-2

ER -