Retardation in somatosensory cortex development induced by postnatal BrdU treatment in mice

Melinda Béldi, J. Takács, G. Bárdos, I. Világi

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1 Citation (Scopus)

Abstract

Cerebral dysgeneses are in the background of several neurological and mental disturbances. The aim of the present study was to investigate structural and activity changes following disturbed postnatal neuronal development in mice. Newborn C57Bl6 mice were exposed to 5-bromo-2′-deoxyuridine (BrdU: daily 50 μg/g body weight) during a period between postnatal days P0-P5 or P0-P11, respectively, and neuronal malformation and malfunctioning of somatosensory (barrel field) cortex was analyzed in adolescent animals. Alterations in histological architecture of interneuronal and glial elements were studied and correlated with electrophysiological modifications. Between P30 and P35 days litters underwent ex vivo electrophysiological experiments to examine the changes in basic excitability and in synaptic efficacy. Parallel immunohistochemistry was performed to detect BrdU, GABA and GFAP. There were no BrdU immunopositive cell nuclei in control animals, but marked staining was observed in both BrdU treated groups. Lessening in the number of GABAergic neurons was observed in the treated groups. GFAP immunohistochemical analysis has shown an increased number of activated astroglial cells in treated animals. Reduction of the number of GABAergic neurons was observed in the treated groups. Electrophysiological recordings on cortical slices showed increased excitability in the treated groups.

Original languageEnglish
Pages (from-to)713-721
Number of pages9
JournalInternational Journal of Developmental Neuroscience
Volume26
Issue number7
DOIs
Publication statusPublished - Nov 2008

Fingerprint

Somatosensory Cortex
Bromodeoxyuridine
GABAergic Neurons
Therapeutics
Cell Nucleus
Neuroglia
gamma-Aminobutyric Acid
Immunohistochemistry
Body Weight
Staining and Labeling

Keywords

  • Brain slice electrophysiology
  • BrdU
  • Development
  • GABA
  • Glial fibrillary acidic protein
  • Immunohistochemistry

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

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abstract = "Cerebral dysgeneses are in the background of several neurological and mental disturbances. The aim of the present study was to investigate structural and activity changes following disturbed postnatal neuronal development in mice. Newborn C57Bl6 mice were exposed to 5-bromo-2′-deoxyuridine (BrdU: daily 50 μg/g body weight) during a period between postnatal days P0-P5 or P0-P11, respectively, and neuronal malformation and malfunctioning of somatosensory (barrel field) cortex was analyzed in adolescent animals. Alterations in histological architecture of interneuronal and glial elements were studied and correlated with electrophysiological modifications. Between P30 and P35 days litters underwent ex vivo electrophysiological experiments to examine the changes in basic excitability and in synaptic efficacy. Parallel immunohistochemistry was performed to detect BrdU, GABA and GFAP. There were no BrdU immunopositive cell nuclei in control animals, but marked staining was observed in both BrdU treated groups. Lessening in the number of GABAergic neurons was observed in the treated groups. GFAP immunohistochemical analysis has shown an increased number of activated astroglial cells in treated animals. Reduction of the number of GABAergic neurons was observed in the treated groups. Electrophysiological recordings on cortical slices showed increased excitability in the treated groups.",
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