Restriction mapping of DNA of temperate Rhizobium meliloti phage 16-3: Comparison of genetic and physical maps indicates a long, genetically silent chromosomal arm

Géza Dallmann, L. Orosz, Béla Sain

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The complete restriction map of DNA (61.57 Kb) of temperate Rhizobium meliloti phage 16-3 has been constructed for enzymes BglII, HindIII, HpaI, KpnI, and a partial map for EcoRI. The strategy employed for mapping included the analysis of double, triple, and partial digests; comparison of wild type and deletion mutants; and detailed analysis of subfragments, exploiting the presence of cohesive ends of the phage. Comparison of the genetic and physical maps indicates that one arm of the chromosome is genetically silent and/or contains nonessential genes.

Original languageEnglish
Pages (from-to)439-448
Number of pages10
JournalMGG Molecular & General Genetics
Volume176
Issue number3
DOIs
Publication statusPublished - Nov 1979

Fingerprint

Sinorhizobium meliloti
Restriction Mapping
Bacteriophages
DNA
Chromosomes
Enzymes
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

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