Restriction fragment length polymorphisms (RFLPs) were detected in the mitochondrial and nuclear DNAs of isolates belonging to the Aspergillus niger aggregate. These strains can be classified into two well-defined groups according to the electrophoretic patterns of their mtDNAs obtained with some restriction enzymes, such as EcoR I, Pvu II, Hae III and Bgl II. Strains belonging to both groups show some further minor variation. Estimates of the molecular weight of these mitochondrial DNAs were between 30 and 32·5 kb. The patterns produced by the nuclear ribosomal DNA repeats in digests of total DNA made it possible to classify the black Aspergillus strains into two groups, each further subdivided into two. The two main groups established from the mitochondrial and ribosomal DNA patterns coincide with each other. A rapid and easy method was also worked out to classify large numbers of field isolates of black aspergilli into one of the groups described using mtDNA patterns obtained by the double-digestion of total DNA samples with Hae III and Bgl II.
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Plant Science