Restraint stress in rats alters gene transcription and protein translation in the hippocampus

Petra Sántha, M. Pákáski, Örsike Csilla Fazekas, Eszter Klára Fodor, J. Kálmán, Z. Janka, Gyula Szabó

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Stress is a relatively new and emerging risk factor for Alzheimer's disease (AD). Severe stress can alter brain characteristics such as neuronal plasticity, due to changes in the metabolism of cytoskeletal proteins. In this study, male Wistar rats were exposed to restraint stress (RS) for 5 h daily for different time periods. At the end of the exposure periods, the amounts of β-actin, cofilin, amyloid precursor protein (APP) and mitogen-activated protein kinase 1 (MAPK-1) RNAs and proteins were investigated. The mRNA expressions of β-actin, cofilin and MAPK-1 followed U-shaped time course. Acute (3 days) and chronic (21 days) RS caused a fourfold and tenfold increases, respectively, in hippocampal β-actin mRNA expression. In the case of cofilin mRNA expression, elevations were detected in the hippocampus on days 3, 7 and 21. The APP mRNA level was increased on day 21. On protein level, chronic stress elevated the levels of β-actin, cofilin and APP in the hippocampus. These results suggest that stress causes the induction of some genes and proteins that are also elevated in AD selectively in the hippocampal region of the rat brain.

Original languageEnglish
Pages (from-to)958-964
Number of pages7
JournalNeurochemical Research
Volume37
Issue number5
DOIs
Publication statusPublished - May 2012

Fingerprint

Actin Depolymerizing Factors
Protein Biosynthesis
Transcription
Rats
Actins
Amyloid beta-Protein Precursor
Hippocampus
Genes
Messenger RNA
Mitogen-Activated Protein Kinase 1
Alzheimer Disease
Proteins
Neuronal Plasticity
Cytoskeletal Proteins
Brain
Wistar Rats
RNA
Metabolism
Plasticity

Keywords

  • β-Actin
  • Alzheimer's disease
  • Amyloid precursor protein
  • Cofilin
  • Mitogen-activated protein kinase 1
  • Stress

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Biochemistry

Cite this

Restraint stress in rats alters gene transcription and protein translation in the hippocampus. / Sántha, Petra; Pákáski, M.; Fazekas, Örsike Csilla; Fodor, Eszter Klára; Kálmán, J.; Janka, Z.; Szabó, Gyula.

In: Neurochemical Research, Vol. 37, No. 5, 05.2012, p. 958-964.

Research output: Contribution to journalArticle

Sántha, Petra ; Pákáski, M. ; Fazekas, Örsike Csilla ; Fodor, Eszter Klára ; Kálmán, J. ; Janka, Z. ; Szabó, Gyula. / Restraint stress in rats alters gene transcription and protein translation in the hippocampus. In: Neurochemical Research. 2012 ; Vol. 37, No. 5. pp. 958-964.
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