Responses to in vitro and in vivo immunisations with Aeromonas salmonicida O antigen bacterins in rainbow trout (Oncorhynchus mykiss)

Douglas P. Anderson, Galina Jeney

Research output: Contribution to journalArticle

8 Citations (Scopus)


Immunological assays were developed to test for the effects of immunisation with Aeromonas salmonicida O antigen bacterin in rainbow trout (Oncorhynchus mykiss) in vitro and in vivo. Trout spleen sections were immunised in vitro by incubation in media containing 100, 10, and 1 μg ml-1 of the A. salmonicida O antigen. After 10 days at 15°C, the individual sections were minced and cell suspensions assayed for the effects of the bacterin on non-specific and specific immune responses. Assays of the cell suspensions showed that the neutrophil oxidative activity, phagocytic capabilities, numbers of plaque-forming cells (PFC) and circulating antibody titres all increased in proportion to the bacterin dosage. Trout immunised in vivo with 100 μg by injection and by 100 μg ml-1 bath with the O antigen, showed similar responses to spleen sections immunised in vitro. The appearance of non-specific PFC against unlabelled sheep red blood cells was highest in spleens immunised in vitro and in the fish immunised by injection; non-specific PFC rarely occurred in O antigen bathed fish and in control fish. The development of these assays may enable biologists to follow the effects on the non-specific defence mechanisms and the specific immune response in cultivated fish populations after, immunisation with A. salmonicida bacterins and thus aid in the development of protective vaccination against furunculosis.

Original languageEnglish
Pages (from-to)251-260
Number of pages10
JournalFish and Shellfish Immunology
Issue number4
Publication statusPublished - Oct 1991



  • Aeromonas salmonicida
  • neutrophil
  • non-specific
  • phagocytosis
  • plaque-forming cells
  • rainbow trout

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

Cite this