Responses of glutathione and glutathione S-transferase to cadmium and mercury exposure in pedunculate oak (Quercus robur) leaf discs

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Abstract

Changes in chlorophyll, non-protein thiol and glutathione (GSH) levels, and the activity of glutathione S-transferase (GST) were investigated in cadmium(II) and mercury(II) chloride treated leaf discs of mature pedunculate oak trees (Quercus robur). Both heavy metals caused decreases in chlorophyll content, but mercury was more toxic than cadmium. Cadmium treatments (30-250 μM) resulted in increasing non-protein thiol levels after 3d, but GSH contents decreased. Mercury (1-20 μM) led to a concentration-dependent decline in both non-protein thiol and GSH levels. GST activities were not modified significantly by cadmium, but mercury treatments caused a dose- and time-dependent enzyme induction. Both the phytotoxic- and GST-inducing effect of mercury could be prevented by the cysteine precursor L-2-oxo-4-thiazolidinecarboxylic acid.

Original languageEnglish
Pages (from-to)62-65
Number of pages4
JournalBotanica Acta
Volume111
Issue number1
Publication statusPublished - Feb 1998

Fingerprint

Quercus robur
glutathione transferase
mercury
glutathione
cadmium
Quercus
thiols
leaves
chlorophyll
gene induction
cysteine
chlorides
heavy metals
acids
dosage

Keywords

  • Cadmium
  • Glutathione
  • Glutathione S-transferase
  • L-2-oxo-4-thiazolidinecarboxylic acid
  • Mercury
  • Oxidative stress
  • Pedunculate oak

ASJC Scopus subject areas

  • Plant Science

Cite this

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title = "Responses of glutathione and glutathione S-transferase to cadmium and mercury exposure in pedunculate oak (Quercus robur) leaf discs",
abstract = "Changes in chlorophyll, non-protein thiol and glutathione (GSH) levels, and the activity of glutathione S-transferase (GST) were investigated in cadmium(II) and mercury(II) chloride treated leaf discs of mature pedunculate oak trees (Quercus robur). Both heavy metals caused decreases in chlorophyll content, but mercury was more toxic than cadmium. Cadmium treatments (30-250 μM) resulted in increasing non-protein thiol levels after 3d, but GSH contents decreased. Mercury (1-20 μM) led to a concentration-dependent decline in both non-protein thiol and GSH levels. GST activities were not modified significantly by cadmium, but mercury treatments caused a dose- and time-dependent enzyme induction. Both the phytotoxic- and GST-inducing effect of mercury could be prevented by the cysteine precursor L-2-oxo-4-thiazolidinecarboxylic acid.",
keywords = "Cadmium, Glutathione, Glutathione S-transferase, L-2-oxo-4-thiazolidinecarboxylic acid, Mercury, Oxidative stress, Pedunculate oak",
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TY - JOUR

T1 - Responses of glutathione and glutathione S-transferase to cadmium and mercury exposure in pedunculate oak (Quercus robur) leaf discs

AU - Gullner, G.

AU - Uotila, M.

AU - Kőmíves, T.

PY - 1998/2

Y1 - 1998/2

N2 - Changes in chlorophyll, non-protein thiol and glutathione (GSH) levels, and the activity of glutathione S-transferase (GST) were investigated in cadmium(II) and mercury(II) chloride treated leaf discs of mature pedunculate oak trees (Quercus robur). Both heavy metals caused decreases in chlorophyll content, but mercury was more toxic than cadmium. Cadmium treatments (30-250 μM) resulted in increasing non-protein thiol levels after 3d, but GSH contents decreased. Mercury (1-20 μM) led to a concentration-dependent decline in both non-protein thiol and GSH levels. GST activities were not modified significantly by cadmium, but mercury treatments caused a dose- and time-dependent enzyme induction. Both the phytotoxic- and GST-inducing effect of mercury could be prevented by the cysteine precursor L-2-oxo-4-thiazolidinecarboxylic acid.

AB - Changes in chlorophyll, non-protein thiol and glutathione (GSH) levels, and the activity of glutathione S-transferase (GST) were investigated in cadmium(II) and mercury(II) chloride treated leaf discs of mature pedunculate oak trees (Quercus robur). Both heavy metals caused decreases in chlorophyll content, but mercury was more toxic than cadmium. Cadmium treatments (30-250 μM) resulted in increasing non-protein thiol levels after 3d, but GSH contents decreased. Mercury (1-20 μM) led to a concentration-dependent decline in both non-protein thiol and GSH levels. GST activities were not modified significantly by cadmium, but mercury treatments caused a dose- and time-dependent enzyme induction. Both the phytotoxic- and GST-inducing effect of mercury could be prevented by the cysteine precursor L-2-oxo-4-thiazolidinecarboxylic acid.

KW - Cadmium

KW - Glutathione

KW - Glutathione S-transferase

KW - L-2-oxo-4-thiazolidinecarboxylic acid

KW - Mercury

KW - Oxidative stress

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