Response of human neutrophil granulocytes to the hyphae of the emerging fungal pathogen curvularia lunata

Eszter Judit Tóth, Mónika Varga, Miklós Takó, Mónika Homa, Olivér Jáger, Edit Hermesz, Hajnalka Orvos, Gábor Nagy, Csaba Vágvölgyi, Tamás Papp

Research output: Contribution to journalArticle

Abstract

Curvularia lunata is an ascomycete filamentous fungus causing local and invasive phaeohyphomycoses in both immunocompromised and immunocompetent patients. Neutrophils are crucial participants of the first line host defense against fungal infections. They migrate to the infected site and eliminate the infectious agents by various mechanisms including phagocytoses, oxidative damage, or formation of neutrophil extracellular trap (NET). Neutropenia may be a risk factor for phaeohyphomycoses, and restoration of the neutrophil function can improve the outcome of the infection. In the present study, interaction of primary human neutrophil granulocytes with the hyphae C. lunata was examined and compared to that with the well characterized filamentous fungal pathogen Aspergillus fumigatus. Neutrophils could recognize the serum opsonized hyphae of C. lunata and attach to them. Myeloperoxidase release was also activated by a soluble factor present in the culture supernatant of the fungus. Induction of the oxidative burst was found to depend on serum opsonization of the hyphae. Although extracellular hydrogen peroxide production was induced, the fungus efficiently blocked the oxidative burst by acidifying the reaction environment. This blockage also affected the NET forming ability of the neutrophils.

Original languageEnglish
Article number235
JournalPathogens
Volume9
Issue number3
DOIs
Publication statusPublished - Mar 2020

Keywords

  • Acidification
  • Curvularia
  • Hydrogen peroxide
  • Myeloperoxidase
  • Neutrophil extracellular trap
  • Oxidative burst
  • Phaeohyphomycosis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Molecular Biology
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

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