Fluorescence spectra were determined at -196° in chloroplasts of greening maize leaves in order to analyse the structural characteristics of the spectra. Digitonin-treated mature chloroplasts were also investigated. Difference spectra calculated from the emission spectra measured at different stages of greening revealed the presence of six fluorescence forms with approximate band positions at 675, 685, 695, 705, 720, and 738 nm respectively. With progressing greening, the minor forms became less discernible, since the spectral composition reached a steady-state. With exception of the form at 705 nm the components reappeared in mature chloroplasts when treated with digitonin.
|Number of pages||6|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - Feb 27 1974|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology