Resolution and retention behaviour of some dyes in overpressured thin-layer chromatography

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Abstract

The performance of conventional and overpressured thin-layer chromatographic techniques was compared in different chamber systems (normal, ultramicro and pressurized chamber), on commercial normal and high-performance thin-layer chromatography (HPTLC) silica gel plates. Data obtained with three dyes demonstrated that separation in the pressurized chamber is similar to those observed in normal and ultramicro chambers, but the diameters of the spots are significantly smaller in separations carried out in pressurized chambers, principally owing to decreased diffusion. It was also observed that the velocity of the moving phase is very stable in the pressurized chamber, and has a linear relationship to solvent flow-rate. Owing to the substantially shorter separation time and stable flow-rates in the pressurized ultramicro chamber, the resolution values obtained on HPTLC plates are also better in the case of longer solvent migration distances.

Original languageEnglish
Pages (from-to)293-300
Number of pages8
JournalJournal of Chromatography A
Volume191
Issue numberC
DOIs
Publication statusPublished - Apr 18 1980

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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