Resolution and reconstitution of the rec BC deoxyribonuclease of Escherichia coli

G. Banfalvi, S. Csuzi, F. Antoni

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The inactivation of rec BC nuclease activity and simultaneously the separation of 3 DNA-dependent ATPases and an ATP-independent DNases specific for single-stranded DNA have been observed after DEAE-cellulose chromatography of cell extracts from Escherichia coli. Two of the ATPases catalyze the strand separation of duplex DNA. Reconstitution of ATP-dependent DNase activity has been carried out by the combination of the separation enzymes.

Original languageEnglish
Pages (from-to)28-32
Number of pages5
JournalFEBS letters
Volume164
Issue number1
DOIs
Publication statusPublished - Nov 28 1983

Keywords

  • ATPase
  • DNase
  • Recombination
  • Unwinding

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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