Resistance to Erysiphe necator in the grapevine 'Kishmish vatkana' is controlled by a single locus through restriction of hyphal growth

Sarolta Hoffmann, Gabriele Di Gaspero, László Kovács, Susanne Howard, E. Kiss, Zsuzsanna Galbács, Raffaele Testolin, Pál Kozma

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Vitis vinifera 'Kishmish vatkana', a cultivated grapevine from Central Asia, does not produce visible symptoms in response to natural or artificial inoculation with the fungus Erysiphe necator Schwein., the casual agent of powdery mildew. 'Kishmish vatkana' allowed pathogen entry into epidermal cells at a rate comparable to that in the susceptible control Vitis vinifera 'Nimrang', but was able to limit subsequent hyphal proliferation. Density of conidiophores was significantly lower in 'Kishmish vatkana' (33.6 ± 8.7 conidiophores mm-2) than in 'Nimrang' (310.5 ± 24.0 conidiophores mm-2) by 120 h after inoculation. A progeny of 310 plants from a 'Nimrang' × 'Kishmish vatkana' cross were scored for the presence or absence of visible conidiophores throughout two successive seasons. Phenotypic segregation revealed the presence of a single dominant allele termed Resistance to Erysiphe necator 1 (REN1), which was heterozygous in 'Kishmish vatkana'. A bulked segregant analysis was carried out using 195 microsatellite markers uniformly distributed across the entire genome. For each marker, association with the resistance trait was inferred by measuring in the bulks the ratio of peak intensities of the two alleles inherited from 'Kishmish vatkana'. The phenotypic locus was assigned to linkage group 13, a genomic region in which no disease resistance had been reported previously. The REN1 position was restricted to a 7.4 cM interval by analyzing the 310 offspring for the segregation of markers that surrounded the target region. The closest markers, VMC9H4-2, VMCNG4E10-1 and UDV-020, were located 0.9 cM away from the REN1 locus.

Original languageEnglish
Pages (from-to)427-438
Number of pages12
JournalTheoretical And Applied Genetics
Volume116
Issue number3
DOIs
Publication statusPublished - Feb 2008

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Necator
Uncinula necator
conidiophores
loci
Vitis
Growth
Vitis vinifera
Alleles
Central Asia
alleles
Disease Resistance
powdery mildew
linkage groups
Microsatellite Repeats
signs and symptoms (plants)
disease resistance
Fungi
Genome
microsatellite repeats
genomics

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science
  • Genetics
  • Genetics(clinical)

Cite this

Resistance to Erysiphe necator in the grapevine 'Kishmish vatkana' is controlled by a single locus through restriction of hyphal growth. / Hoffmann, Sarolta; Di Gaspero, Gabriele; Kovács, László; Howard, Susanne; Kiss, E.; Galbács, Zsuzsanna; Testolin, Raffaele; Kozma, Pál.

In: Theoretical And Applied Genetics, Vol. 116, No. 3, 02.2008, p. 427-438.

Research output: Contribution to journalArticle

Hoffmann, Sarolta ; Di Gaspero, Gabriele ; Kovács, László ; Howard, Susanne ; Kiss, E. ; Galbács, Zsuzsanna ; Testolin, Raffaele ; Kozma, Pál. / Resistance to Erysiphe necator in the grapevine 'Kishmish vatkana' is controlled by a single locus through restriction of hyphal growth. In: Theoretical And Applied Genetics. 2008 ; Vol. 116, No. 3. pp. 427-438.
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AU - Kovács, László

AU - Howard, Susanne

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AU - Kozma, Pál

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