Research note: Identification of the gene product recognized by monoclonal antibody GIIF3

B. Felfoldi, A. Magyar, N. Nagy, I. Olah, G. T. Pharr

Research output: Contribution to journalArticle

Abstract

The chicken as a research model has a disadvantage compared with the mouse and the human because of the low number of available antibodies against gene products of interest. The goal of this study was to identify the antigen recognized by monoclonal antibody (mAb) GIIF3, which is a 42 kDa protein that appears in follicle-associated epithelium of the Guinea hen as well as in different muscle types during chicken embryonic development. The 42 kDa protein, immunoprecipitated from chicken gizzard protein lysates, was evaluated by mass spectrometry. Mass spectrometry analysis revealed peptides specific for the chicken β- or γ-actin isoforms. The mAb GIIF3 can be used as a new research tool for smooth muscle cell and bursa of Fabricius developmental studies.

Original languageEnglish
Pages (from-to)474-477
Number of pages4
JournalPoultry science
Volume96
Issue number2
DOIs
Publication statusPublished - Jan 1 2017

Keywords

  • Chicken gizzard
  • GIIF3
  • Monoclonal antibody
  • Smooth muscle actin

ASJC Scopus subject areas

  • Animal Science and Zoology

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