Rescue of injured motoneurones by grafted neuroectodermal stem cells: effect of the location of graft.

Gholam Pajenda, Krisztián Pajer, Gábor Márton, P. Hegyi, Heinz Redl, Antal Nógrádi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Avulsion of one or more ventral roots from the spinal cord leads to the death of the majority of affected motoneurons. In this study we investigated whether immortalized clonal neuroectodermal stem cells applied to the injured cord in various ways impart neuroprotection on motoneurons otherwise destined to die. The lumbar 4 (L4) ventral root of Sprague-Dawley rats was avulsed and reimplanted ventrolaterally into the injured cord. Clonal neuroectodermal murine stem cells (NE-GFP-4C) were placed in fibrin clot around the reimplanted root, were injected immediately following avulsion into the reimplanted ventral root or directly into the L4 segment. Three months after the primary surgery the L4 motoneuron pool was retrogradely labelled with Fast blue and the numbers of reinnervating motoneurons were determined. Functional recovery was tested biweekly through the use of the CatWalk automated gait analysis system. Transplantation of neuroectodermal stem cells into the reimplanted root or into the L4 spinal segment resulted in similarly extensive regeneration of the motoneurons (671 ± 26 and 711 ± 14 L4 motoneurons, respectively). In these groups significant functional recovery was achieved. The negative controls and animals with periradicular stem cell treatment showed poor motor recovery and reinnervation (42 ± 10 and 65 ± 2.5, respectively). This study provides evidence that neuroectodermal stem cell transplantation into the reimplanted ventral root induces as successful regeneration of injured motoneurons as stem cells grafted into the spinal cord.

Original languageEnglish
Pages (from-to)263-274
Number of pages12
JournalRestorative Neurology and Neuroscience
Volume31
Issue number3
Publication statusPublished - 2013

Fingerprint

Motor Neurons
Stem Cells
Transplants
Spinal Nerve Roots
Stem Cell Transplantation
Regeneration
Spinal Cord
Fibrin
Gait
Sprague Dawley Rats

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience
  • Clinical Neurology
  • Medicine(all)

Cite this

Rescue of injured motoneurones by grafted neuroectodermal stem cells : effect of the location of graft. / Pajenda, Gholam; Pajer, Krisztián; Márton, Gábor; Hegyi, P.; Redl, Heinz; Nógrádi, Antal.

In: Restorative Neurology and Neuroscience, Vol. 31, No. 3, 2013, p. 263-274.

Research output: Contribution to journalArticle

Pajenda, Gholam ; Pajer, Krisztián ; Márton, Gábor ; Hegyi, P. ; Redl, Heinz ; Nógrádi, Antal. / Rescue of injured motoneurones by grafted neuroectodermal stem cells : effect of the location of graft. In: Restorative Neurology and Neuroscience. 2013 ; Vol. 31, No. 3. pp. 263-274.
@article{732756344da3488eb632e48fd5cbb7f6,
title = "Rescue of injured motoneurones by grafted neuroectodermal stem cells: effect of the location of graft.",
abstract = "Avulsion of one or more ventral roots from the spinal cord leads to the death of the majority of affected motoneurons. In this study we investigated whether immortalized clonal neuroectodermal stem cells applied to the injured cord in various ways impart neuroprotection on motoneurons otherwise destined to die. The lumbar 4 (L4) ventral root of Sprague-Dawley rats was avulsed and reimplanted ventrolaterally into the injured cord. Clonal neuroectodermal murine stem cells (NE-GFP-4C) were placed in fibrin clot around the reimplanted root, were injected immediately following avulsion into the reimplanted ventral root or directly into the L4 segment. Three months after the primary surgery the L4 motoneuron pool was retrogradely labelled with Fast blue and the numbers of reinnervating motoneurons were determined. Functional recovery was tested biweekly through the use of the CatWalk automated gait analysis system. Transplantation of neuroectodermal stem cells into the reimplanted root or into the L4 spinal segment resulted in similarly extensive regeneration of the motoneurons (671 ± 26 and 711 ± 14 L4 motoneurons, respectively). In these groups significant functional recovery was achieved. The negative controls and animals with periradicular stem cell treatment showed poor motor recovery and reinnervation (42 ± 10 and 65 ± 2.5, respectively). This study provides evidence that neuroectodermal stem cell transplantation into the reimplanted ventral root induces as successful regeneration of injured motoneurons as stem cells grafted into the spinal cord.",
author = "Gholam Pajenda and Kriszti{\'a}n Pajer and G{\'a}bor M{\'a}rton and P. Hegyi and Heinz Redl and Antal N{\'o}gr{\'a}di",
year = "2013",
language = "English",
volume = "31",
pages = "263--274",
journal = "Restorative Neurology and Neuroscience",
issn = "0922-6028",
publisher = "IOS Press",
number = "3",

}

TY - JOUR

T1 - Rescue of injured motoneurones by grafted neuroectodermal stem cells

T2 - effect of the location of graft.

AU - Pajenda, Gholam

AU - Pajer, Krisztián

AU - Márton, Gábor

AU - Hegyi, P.

AU - Redl, Heinz

AU - Nógrádi, Antal

PY - 2013

Y1 - 2013

N2 - Avulsion of one or more ventral roots from the spinal cord leads to the death of the majority of affected motoneurons. In this study we investigated whether immortalized clonal neuroectodermal stem cells applied to the injured cord in various ways impart neuroprotection on motoneurons otherwise destined to die. The lumbar 4 (L4) ventral root of Sprague-Dawley rats was avulsed and reimplanted ventrolaterally into the injured cord. Clonal neuroectodermal murine stem cells (NE-GFP-4C) were placed in fibrin clot around the reimplanted root, were injected immediately following avulsion into the reimplanted ventral root or directly into the L4 segment. Three months after the primary surgery the L4 motoneuron pool was retrogradely labelled with Fast blue and the numbers of reinnervating motoneurons were determined. Functional recovery was tested biweekly through the use of the CatWalk automated gait analysis system. Transplantation of neuroectodermal stem cells into the reimplanted root or into the L4 spinal segment resulted in similarly extensive regeneration of the motoneurons (671 ± 26 and 711 ± 14 L4 motoneurons, respectively). In these groups significant functional recovery was achieved. The negative controls and animals with periradicular stem cell treatment showed poor motor recovery and reinnervation (42 ± 10 and 65 ± 2.5, respectively). This study provides evidence that neuroectodermal stem cell transplantation into the reimplanted ventral root induces as successful regeneration of injured motoneurons as stem cells grafted into the spinal cord.

AB - Avulsion of one or more ventral roots from the spinal cord leads to the death of the majority of affected motoneurons. In this study we investigated whether immortalized clonal neuroectodermal stem cells applied to the injured cord in various ways impart neuroprotection on motoneurons otherwise destined to die. The lumbar 4 (L4) ventral root of Sprague-Dawley rats was avulsed and reimplanted ventrolaterally into the injured cord. Clonal neuroectodermal murine stem cells (NE-GFP-4C) were placed in fibrin clot around the reimplanted root, were injected immediately following avulsion into the reimplanted ventral root or directly into the L4 segment. Three months after the primary surgery the L4 motoneuron pool was retrogradely labelled with Fast blue and the numbers of reinnervating motoneurons were determined. Functional recovery was tested biweekly through the use of the CatWalk automated gait analysis system. Transplantation of neuroectodermal stem cells into the reimplanted root or into the L4 spinal segment resulted in similarly extensive regeneration of the motoneurons (671 ± 26 and 711 ± 14 L4 motoneurons, respectively). In these groups significant functional recovery was achieved. The negative controls and animals with periradicular stem cell treatment showed poor motor recovery and reinnervation (42 ± 10 and 65 ± 2.5, respectively). This study provides evidence that neuroectodermal stem cell transplantation into the reimplanted ventral root induces as successful regeneration of injured motoneurons as stem cells grafted into the spinal cord.

UR - http://www.scopus.com/inward/record.url?scp=84891413304&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891413304&partnerID=8YFLogxK

M3 - Article

C2 - 23478343

AN - SCOPUS:84891413304

VL - 31

SP - 263

EP - 274

JO - Restorative Neurology and Neuroscience

JF - Restorative Neurology and Neuroscience

SN - 0922-6028

IS - 3

ER -