Requirement of zymogen modification for activation of porcine plasminogen

R. Machovich, Robert D. Litwiller, Whyte G. Owen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In physiological salt solutions, porcine plasminogen is refractory to activation by urokinase or trypsin and to proteolysis at Lys77 by plasmin or trypsin. Plasminogen becomes a substrate for urokinase (at Arg560), plasmin (at Lys77), and trypsin (at both bonds) if chloride ion is removed or if 6-aminohexanoate (2.5 mmol/L) is added. Irrespective of salts, activation of des(1-77)plasminogen is as efficient as activation of des(kringle1-4)plasminogen and is inhibited 50% by 2.5 mmol/L 6-aminohexanoate. In solutions lacking chloride or containing 6-aminohexanoate, plasminogen, des(1-77)plasminogen, and des(kringle1-4)plasminogen show no tendency to saturate urokinase in physiologically relevant concentrations (10 μmol/L). The findings are interpreted as indicating that plasminogen requires modification, either by proteolysis or by ligands, for activation.

Original languageEnglish
Pages (from-to)11558-11561
Number of pages4
JournalBiochemistry
Volume31
Issue number46
Publication statusPublished - 1992

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Enzyme Precursors
Plasminogen
Swine
Chemical activation
Aminocaproates
Urokinase-Type Plasminogen Activator
Trypsin
Proteolysis
Fibrinolysin
Chlorides
Salts
Refractory materials
Ions
Ligands
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Machovich, R., Litwiller, R. D., & Owen, W. G. (1992). Requirement of zymogen modification for activation of porcine plasminogen. Biochemistry, 31(46), 11558-11561.

Requirement of zymogen modification for activation of porcine plasminogen. / Machovich, R.; Litwiller, Robert D.; Owen, Whyte G.

In: Biochemistry, Vol. 31, No. 46, 1992, p. 11558-11561.

Research output: Contribution to journalArticle

Machovich, R, Litwiller, RD & Owen, WG 1992, 'Requirement of zymogen modification for activation of porcine plasminogen', Biochemistry, vol. 31, no. 46, pp. 11558-11561.
Machovich, R. ; Litwiller, Robert D. ; Owen, Whyte G. / Requirement of zymogen modification for activation of porcine plasminogen. In: Biochemistry. 1992 ; Vol. 31, No. 46. pp. 11558-11561.
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