Requirement of membrane-proximal amino acids in the carboxyl-terminal tail for expression of the rat AT(1a) angiotensin receptor

Zsuzsanna Gáborik, Balázs Mihalik, Suman Jayadev, Gowraganahalli Jagadeesh, Kevin J. Catt, László Hunyady

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Abstract

A series of deletion mutants was created to analyze the function of the membrane-proximal region of the cytoplasmic tail of the rat type 1a (AT(1a)) angiotensin receptor. In transiently transfected COS-7 cells, the truncated mutant receptors showed a progressive decrease in surface expression, with no major change in binding affinity for the peptide antagonist, |Sar1,lle8| angiotensin II. In parallel with the decrease in receptor expression, a progressive decrease in angiotensin II-induced inositol phosphate responses was observed. Alanine substitutions in the region 307-311 identified the highly conserved phenylalanine309 and adjacent lysine residues as significant determinants of AT(1a) receptor expression.

Original languageEnglish
Pages (from-to)147-151
Number of pages5
JournalFEBS letters
Volume428
Issue number3
DOIs
Publication statusPublished - May 29 1998

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Keywords

  • AT angiotensin receptor
  • Ca mobilizing hormone
  • Carboxyl-terminal tail
  • Inositol phosphate
  • Receptor expression

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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