Requirement of carotene isomerization for the assembly of photosystem II in Synechocystis sp. PCC 6803

Kazumori Masamoto, Shin Ichiro Hisatomi, Isamu Sakurai, Zoltán Gombos, Hajime Wada

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Carotene isomerase mutant (crtH mutant) cells of Synechocystis sp. PCC 6803 can accumulate β-carotene under light conditions. However, the mutant cells grown under a light-activated heterotrophic growth condition contained detectable levels of neither β-carotene nor D1 protein of the photosystem (PS) II reaction center, and no oxygen-evolving activity of PSII was detected. β-Carotene and D1 protein appeared and a high level of PSII activity was detected after the cells were transferred to a continuous light condition. The PSI activities of thylakoid membranes from mutant cells were almost the same as those of thylakoid membranes from wild-type cells, both before and after transfer to the continuous light condition. These results suggest that β-carotene is required for the assembly of PSII but not for that of PSI.

Original languageEnglish
Pages (from-to)1325-1329
Number of pages5
JournalPlant and Cell Physiology
Volume45
Issue number9
DOIs
Publication statusPublished - Sep 2004

Keywords

  • Carotene isomerase
  • Cyanobacterium
  • Photoisomerization
  • Synechocystis sp. PCC 6803
  • cis-Lycopene
  • crtH

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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