Requirement of carotene isomerization for the assembly of photosystem II in Synechocystis sp. PCC 6803

Kazumori Masamoto, Shin Ichiro Hisatomi, Isamu Sakurai, Z. Gombos, Hajime Wada

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Carotene isomerase mutant (crtH mutant) cells of Synechocystis sp. PCC 6803 can accumulate β-carotene under light conditions. However, the mutant cells grown under a light-activated heterotrophic growth condition contained detectable levels of neither β-carotene nor D1 protein of the photosystem (PS) II reaction center, and no oxygen-evolving activity of PSII was detected. β-Carotene and D1 protein appeared and a high level of PSII activity was detected after the cells were transferred to a continuous light condition. The PSI activities of thylakoid membranes from mutant cells were almost the same as those of thylakoid membranes from wild-type cells, both before and after transfer to the continuous light condition. These results suggest that β-carotene is required for the assembly of PSII but not for that of PSI.

Original languageEnglish
Pages (from-to)1325-1329
Number of pages5
JournalPlant and Cell Physiology
Volume45
Issue number9
Publication statusPublished - Sep 2004

Fingerprint

Synechocystis sp. PCC 6803
Synechocystis
Photosystem II Protein Complex
isomerization
Carotenoids
carotenes
photosystem II
Light
mutants
D1 protein
Thylakoids
thylakoids
cells
Heterotrophic Processes
Isomerases
isomerases
Proteins
Oxygen
oxygen

Keywords

  • Carotene isomerase
  • cis-Lycopene
  • crtH
  • Cyanobacterium
  • Photoisomerization
  • Synechocystis sp. PCC 6803

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Requirement of carotene isomerization for the assembly of photosystem II in Synechocystis sp. PCC 6803. / Masamoto, Kazumori; Hisatomi, Shin Ichiro; Sakurai, Isamu; Gombos, Z.; Wada, Hajime.

In: Plant and Cell Physiology, Vol. 45, No. 9, 09.2004, p. 1325-1329.

Research output: Contribution to journalArticle

Masamoto, Kazumori ; Hisatomi, Shin Ichiro ; Sakurai, Isamu ; Gombos, Z. ; Wada, Hajime. / Requirement of carotene isomerization for the assembly of photosystem II in Synechocystis sp. PCC 6803. In: Plant and Cell Physiology. 2004 ; Vol. 45, No. 9. pp. 1325-1329.
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