Replication of bovine herpesvirus type 4 in human cells in vitro

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A reference strain (Movar 33/63) of bovine herpesvirus type 4 (BHV-4) was inoculated into 14 different human cell lines and five primary cell cultures representing various human tissues. BHV-4 replicated in two embryonic lung cell lines, MRC-5 and Wistar-38, and in a giant-cell glioblastoma cell culture. Cytopathic effect and intranuclear inclusion bodies were observed in these cells. PCR detected a 10,000-times-higher level of BHV-4 DNA. Titration of the supernatant indicated a 100-fold increase of infectious particles. Since this is the first bovine (human herpesvirus 8 and Epstein-Barr virus related) herpesvirus which replicates on human cells in vitro, the danger of possible human BHV-4 infection should not be ignored.

Original languageEnglish
Pages (from-to)2109-2111
Number of pages3
JournalJournal of Clinical Microbiology
Issue number7
Publication statusPublished - 1998

ASJC Scopus subject areas

  • Microbiology (medical)

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