Repeated epicutaneous exposures to ovalbumin progressively induce atopic dermatitis-like skin lesions in mice

G. Wang, T. Savinko, H. Wolff, M. C. Dieu-Nosjean, L. Kemény, B. Homey, A. I. Lauerma, H. Alenius

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background: Atopic dermatitis (AD) is a chronic skin disease in which environmental factors play a great role. A widely used murine model for AD has provided a useful tool to study the disease. Objective: The purpose of this study is to investigate kinetically the induction of this AD model and the processes involved in the development of AD due to extrinsic allergen exposures. Methods: BALB/c mice were epicutaneously exposed to ovalbumin (OVA) for 3 weeks; each week was separated by a 2-week resting period. Mice were killed after each exposure week. Skin biopsies and blood were obtained for histological study, RNA isolation and antibody analysis. Results: There was a progressive and significant thickening of the epidermis and dermis in OVA-exposed mice. Significantly increased dermal cell infiltration of eosinophils, mast cells and total inflammatory cells, including CD3 and CD4 cells, was found after each OVA exposure week. Total IgE, IgG2a and OVA-specific IgE were significantly increased after the second and third exposure week, while OVA-specific IgG2a was significantly induced after the third exposure week. Gradual and/or significant increases in mRNA expression of IL-1β, TNF-α, IL-4, IL-10, IL-13, IFN-γ and IL-12p35 were found after each exposure week. Chemokines and their receptors involved in both T-helper type 1 (Th1)- and Th2-type cell recruitment (CCL1, CCL8, CCL11, CCL24, CXCL9, CXCL10, CCR1, CCR3, CCR5, CCR8 and CXCR3) were up-regulated significantly at different time-points. Conclusion: This study provides an insight into the dynamic nature and time-dependent transition of skin inflammation and systemic immune responses in a murine AD model induced by repeated epicutaneous exposures to OVA.

Original languageEnglish
Pages (from-to)151-161
Number of pages11
JournalClinical and Experimental Allergy
Volume37
Issue number1
DOIs
Publication statusPublished - Jan 2007

Fingerprint

Ovalbumin
Atopic Dermatitis
Skin
Immunoglobulin E
Interleukin-12 Subunit p35
Th2 Cells
Th1 Cells
Interleukin-13
Chemokine Receptors
Dermis
Interleukin-1
Skin Diseases
Eosinophils
Epidermis
Mast Cells
Interleukin-4
Interleukin-10
Allergens
Chronic Disease
RNA

Keywords

  • Animal model
  • Atopic dermatitis
  • Chemokine receptors
  • Chemokines
  • Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

Repeated epicutaneous exposures to ovalbumin progressively induce atopic dermatitis-like skin lesions in mice. / Wang, G.; Savinko, T.; Wolff, H.; Dieu-Nosjean, M. C.; Kemény, L.; Homey, B.; Lauerma, A. I.; Alenius, H.

In: Clinical and Experimental Allergy, Vol. 37, No. 1, 01.2007, p. 151-161.

Research output: Contribution to journalArticle

Wang, G. ; Savinko, T. ; Wolff, H. ; Dieu-Nosjean, M. C. ; Kemény, L. ; Homey, B. ; Lauerma, A. I. ; Alenius, H. / Repeated epicutaneous exposures to ovalbumin progressively induce atopic dermatitis-like skin lesions in mice. In: Clinical and Experimental Allergy. 2007 ; Vol. 37, No. 1. pp. 151-161.
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