Renaturation of citrate synthase: Influence of denaturant and folding assistants

W. Zhi, S. J. Landry, L. M. Gierasch, P. Srere

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88 Citations (Scopus)

Abstract

Citrate synthase (CS), which has been denatured in either guanidine hydrochloride (GdnHCl) or urea can be assisted in its renaturation in a variety of ways. The addition of each of the assistants - bovine serum albumin (BSA), oxaloacetate (OAA), and glycerol - promotes renaturation. In combination, the effect of these substances is additive with respect to the yield of folded CS. The report of Buchner et al. (Buchner, J., Schmidt, M., Fuchs, M., Jaenicke, R., Rudolph, R., Schmid, F.X., and Kiefhaber, T., 1991, Biochemistry 30, 1586-1591) that refolding of CS is facilitated by the GroE system (an Escherichia coli chaperonin [cpn] that is composed of GroEL [cpn60] and GroES [cpn10]) has been confirmed. However, we observed substantially higher yield of reactivated CS, 82%, and almost no reactivation in the absence of GroES,

Original languageEnglish
Pages (from-to)522-529
Number of pages8
JournalProtein Science
Volume1
Issue number4
Publication statusPublished - 1992

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Citrate (si)-Synthase
Chaperonins
Oxaloacetic Acid
Biochemistry
Guanidine
Bovine Serum Albumin
Glycerol
Escherichia coli
Urea

ASJC Scopus subject areas

  • Biochemistry

Cite this

Renaturation of citrate synthase : Influence of denaturant and folding assistants. / Zhi, W.; Landry, S. J.; Gierasch, L. M.; Srere, P.

In: Protein Science, Vol. 1, No. 4, 1992, p. 522-529.

Research output: Contribution to journalArticle

Zhi, W, Landry, SJ, Gierasch, LM & Srere, P 1992, 'Renaturation of citrate synthase: Influence of denaturant and folding assistants', Protein Science, vol. 1, no. 4, pp. 522-529.
Zhi, W. ; Landry, S. J. ; Gierasch, L. M. ; Srere, P. / Renaturation of citrate synthase : Influence of denaturant and folding assistants. In: Protein Science. 1992 ; Vol. 1, No. 4. pp. 522-529.
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