Renal nitric oxide production in rat pregnancy: Role of constitutive nitric oxide synthases

Cheryl A. Smith, Beth Santymire, Aaron Erdely, Vasuki Venkat, G. Losonczy, Chris Baylis

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Functional studies show that increased renal nitric oxide (NO) mediates the renal vasodilation and increased glomerular filtration rate that occur during normal pregnancy. We investigated whether changes in the constitutive NO synthases (NOS), endothelial (eNOS) and neuronal (nNOS), were associated with the increased renal NO production in normal midterm pregnancy in the rat. In kidneys from midterm pregnant (MP: 11-13 days gestation), late-term pregnant (LP: 18-20 days gestation), and similarly aged virgin (V) rats, transcript and protein abundance for eNOS and the nNOSα and nNOSβ splice variants, as well as the rate of L-arginine-to-L-citrulline conversion, were determined as a measure of NOS activity. At MP, renal cortical abundance of the total eNOS protein and phosphorylated (Ser1177) eNOS was reduced, and L-arginine-to-L-citrulline conversion in the cortical membrane fraction was decreased; these declines were also seen in LP. There were no changes in the eNOS transcript. In contrast, L-arginine-to-L-citrulline conversion in the soluble fraction of renal cortex increased at MP and then declined at LP. This MP increase was ablated by S-methylthiocitrulline, a nNOS inhibitor. Using Western blotting, we did not detect a change in the protein abundance or transcript of the 160-kDa nNOSα, but protein abundance and transcript of the nNOSβ were increased at MP in cortex. Collectively, these studies suggest that the soluble nNOSβ is responsible for the increased renal cortical NO production during pregnancy.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Physiology
Volume299
Issue number4
DOIs
Publication statusPublished - Oct 2010

Fingerprint

Nitric Oxide Synthase
Nitric Oxide
Kidney
Pregnancy
Citrulline
Arginine
Proteins
Nitric Oxide Synthase Type III
Glomerular Filtration Rate
Vasodilation
Western Blotting
Membranes

Keywords

  • Endothelial nitric oxide synthase
  • L-arginine-to-L-citrulline conversion
  • Neuronal nitric oxide synthase
  • S-methylthiocitrulline
  • Splice variants

ASJC Scopus subject areas

  • Physiology
  • Urology
  • Medicine(all)

Cite this

Renal nitric oxide production in rat pregnancy : Role of constitutive nitric oxide synthases. / Smith, Cheryl A.; Santymire, Beth; Erdely, Aaron; Venkat, Vasuki; Losonczy, G.; Baylis, Chris.

In: American Journal of Physiology - Renal Physiology, Vol. 299, No. 4, 10.2010.

Research output: Contribution to journalArticle

Smith, Cheryl A. ; Santymire, Beth ; Erdely, Aaron ; Venkat, Vasuki ; Losonczy, G. ; Baylis, Chris. / Renal nitric oxide production in rat pregnancy : Role of constitutive nitric oxide synthases. In: American Journal of Physiology - Renal Physiology. 2010 ; Vol. 299, No. 4.
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