Removal of nonspecific binding proteins from cell and tissue extracts using 2-aminobenzimidazole-tethered affinity resin

E. Molnár, G. Fábián, J. Klem, Z. Darula, E. Hunyadi-Gulyás, Á Medgyesi, K. F. Medzihradszky, L. Puskás

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cellular drug target identification through affinity chromatography is often hindered by the quantity of nonspecific binders, such as cytoskeletal and heat shock proteins. Thus, we prepared a 2-aminobenzimidazole-tethered depletion resin designed for removal of these proteins, and tested it on human lung carcinoma cell and rat tissue extracts. Column-bound proteins were identified by twodimensional gel electrophoresis and MS. Among others, tubulins, actins and heat shock proteins were successfully depleted. Due to the reduction of these highly abundant proteins detection of potential drug targets is considerably facilitated in the pre-purified samples.

Original languageEnglish
Pages (from-to)662-665
Number of pages4
JournalPharmazie
Volume66
Issue number9
DOIs
Publication statusPublished - Sep 2011

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Tissue Extracts
Cell Extracts
Carrier Proteins
Heat-Shock Proteins
Proteins
Tubulin
Affinity Chromatography
Pharmaceutical Preparations
Electrophoresis
Actins
Gels
Carcinoma
Lung
2-aminobenzimidazole

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Removal of nonspecific binding proteins from cell and tissue extracts using 2-aminobenzimidazole-tethered affinity resin. / Molnár, E.; Fábián, G.; Klem, J.; Darula, Z.; Hunyadi-Gulyás, E.; Medgyesi, Á; Medzihradszky, K. F.; Puskás, L.

In: Pharmazie, Vol. 66, No. 9, 09.2011, p. 662-665.

Research output: Contribution to journalArticle

Molnár, E. ; Fábián, G. ; Klem, J. ; Darula, Z. ; Hunyadi-Gulyás, E. ; Medgyesi, Á ; Medzihradszky, K. F. ; Puskás, L. / Removal of nonspecific binding proteins from cell and tissue extracts using 2-aminobenzimidazole-tethered affinity resin. In: Pharmazie. 2011 ; Vol. 66, No. 9. pp. 662-665.
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