Removal of lysed blastomeres from frozen-thawed embryos improves implantation and pregnancy rates in frozen embryo transfer cycles

P. Nagy, Tyl Taylor, Thomas Elliott, Joe B. Massey, Hilton I. Kort, Daniel B. Shapiro

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective: To evaluate the effect of degenerated (lysed) blastomere removal on implantation and pregnancy rates in cleavage-stage cryo-embryo transfer (ET) cycles. Design: Randomized clinical trial. Setting: Private reproductive medical center. Patient(s): A total of 88 patients who received frozen-thawed ET, divided into two groups. Intervention(s): Embryo freezing and thawing; opening of the zona pellucida and removal of cryodamaged blastomeres (in the study group), followed by same-day ET. Main Outcome Measure(s): Extent of survival of cleavage-stage embryos after the freeze-thaw procedure; embryo implantation and clinical pregnancies. Result(s): Oocyte number per patient, fertilization rate, embryo development rate (and quality), and freezing rates were similar in the two groups in the fresh cycle. In the control group, a total of 55 embryos (25%) of the 217 thawed remained fully intact, and 53 (26%) of the 207 in the study group remained intact. The average number of embryos transferred per group was similar (control, 3.4 ± 0.9; study, 3.3 ± 0.9). Implantation rates were 12% and 26% in the control and study groups, respectively. The clinical pregnancy rate was 23% in the control group and 64% in the study group when lysed cell removal was performed. Conclusion(s): The results show that pregnancy and implantation rates are higher in the study group; therefore, the removal of degenerated blastomeres may be beneficial to all patients who undergo cleavage-stage, frozen-thawed ET.

Original languageEnglish
Pages (from-to)1606-1612
Number of pages7
JournalFertility and Sterility
Volume84
Issue number6
DOIs
Publication statusPublished - Dec 2005

Fingerprint

Blastomeres
Embryo Transfer
Pregnancy Rate
Embryonic Structures
Control Groups
Freezing
Zona Pellucida
Fertilization
Oocytes
Embryonic Development
Randomized Controlled Trials
Outcome Assessment (Health Care)
Pregnancy
Survival

Keywords

  • Assisted hatching
  • Cryopreservation
  • Embryo
  • Implantation
  • Pregnancy

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Removal of lysed blastomeres from frozen-thawed embryos improves implantation and pregnancy rates in frozen embryo transfer cycles. / Nagy, P.; Taylor, Tyl; Elliott, Thomas; Massey, Joe B.; Kort, Hilton I.; Shapiro, Daniel B.

In: Fertility and Sterility, Vol. 84, No. 6, 12.2005, p. 1606-1612.

Research output: Contribution to journalArticle

Nagy, P. ; Taylor, Tyl ; Elliott, Thomas ; Massey, Joe B. ; Kort, Hilton I. ; Shapiro, Daniel B. / Removal of lysed blastomeres from frozen-thawed embryos improves implantation and pregnancy rates in frozen embryo transfer cycles. In: Fertility and Sterility. 2005 ; Vol. 84, No. 6. pp. 1606-1612.
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abstract = "Objective: To evaluate the effect of degenerated (lysed) blastomere removal on implantation and pregnancy rates in cleavage-stage cryo-embryo transfer (ET) cycles. Design: Randomized clinical trial. Setting: Private reproductive medical center. Patient(s): A total of 88 patients who received frozen-thawed ET, divided into two groups. Intervention(s): Embryo freezing and thawing; opening of the zona pellucida and removal of cryodamaged blastomeres (in the study group), followed by same-day ET. Main Outcome Measure(s): Extent of survival of cleavage-stage embryos after the freeze-thaw procedure; embryo implantation and clinical pregnancies. Result(s): Oocyte number per patient, fertilization rate, embryo development rate (and quality), and freezing rates were similar in the two groups in the fresh cycle. In the control group, a total of 55 embryos (25{\%}) of the 217 thawed remained fully intact, and 53 (26{\%}) of the 207 in the study group remained intact. The average number of embryos transferred per group was similar (control, 3.4 ± 0.9; study, 3.3 ± 0.9). Implantation rates were 12{\%} and 26{\%} in the control and study groups, respectively. The clinical pregnancy rate was 23{\%} in the control group and 64{\%} in the study group when lysed cell removal was performed. Conclusion(s): The results show that pregnancy and implantation rates are higher in the study group; therefore, the removal of degenerated blastomeres may be beneficial to all patients who undergo cleavage-stage, frozen-thawed ET.",
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