Reliability of methods of estimation of genetic distance in barley (Hordeum vulgare L.)

K. Mészáros, I. Karsai, P. M. Hayes, Z. Bedö

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The reliability of genetic distance estimations based on the RFLP method, was evaluated in nine barley varieties. Varieties with differing growth habits could be distinguished with the aid of the RFLP probes. Within the winter barleys, the two- and six-row types were quite distinct. The six-row winter barleys could be grouped according to site of origin and known degree of relationship. The Jaccard distances obtained on the basis of individual RFLP band numbers exhibited close correlation (r = 0.946, p = 0.001) with those obtained by evaluating patterns within groups, but differences were observed within groups of varieties closely related to each other. These differences arose from different interpretations of the complex probes; however, this did not lead to significant distortions. In tests of four varieties, the Jaccard distances obtained with 24 or 65 RFLP probes did not differ substantially, as shown by the close correlation between them (r = 0.968). The results obtained when the number of probes was almost tripled did not differ from those obtained with 24 probes and did not provide more information on the degree of relationship. The evaluation of the patterns obtained with 24 RFLP probes was found to be simpler than that involving individual band numbers and gave a reliable estimation of the degree of relationship between barley varieties.

Original languageEnglish
Pages (from-to)387-392
Number of pages6
JournalJournal of Genetics and Breeding
Issue number4
Publication statusPublished - Dec 1 1996



  • Barley
  • Genetic distance
  • RFLP

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Horticulture

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