Release of potassium, lipids, and proteins from nonionic detergent treated chicken red blood cells

Miklós Kellermayer, Andrea Ludány, A. Miseta, T. Kőszegi, Gábor Berta, P. Bogner, Carlton F. Hazlewood, Ivan L. Cameron, Denys N. Wheatley

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The plasma membrane of erythrocytes, as of other cells, is thought to act as the barrier responsible for maintaining intracellular gradients of most ions and small molecular species between the cell and its environment. Controlled application of the nonionic detergent Brij 58 effectively opened the erythrocyte plasma membrane, as judged by electron microscopy and lipid mobilization, but the cytoplasm maintained much of its integrity for about 30 min. Release of K+ correlated well with release of protein into the surrounding medium. The results demonstrate that permeabilization of the erythrocyte plasma membrane does not result in an instantaneous equilibration of small ions, such as K+, between the cell and its environment. A comparison was made between erythrocytes treated with Brij 58 and Triton X-100. The lipid and protein solubilizing actions of Triton X-100 were not as easily separable in time as those of Brij 58. The results of treatment of the erythrocytes with different types of nonionic detergents suggest that the membranolytic and cytoplasmic protein destabilizing actions of nonionic detergents correspond with their hydrophilic-lipophilic balance numbers (HLB values).

Original languageEnglish
Pages (from-to)197-204
Number of pages8
JournalJournal of Cellular Physiology
Volume159
Issue number2
Publication statusPublished - May 1994

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Cetomacrogol
Cell membranes
Detergents
Chickens
Potassium
Blood
Erythrocytes
Cells
Octoxynol
Cell Membrane
Erythrocyte Membrane
Lipids
Lipid Mobilization
Ions
Proteins
Electron microscopy
Electron Microscopy
Cytoplasm

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Release of potassium, lipids, and proteins from nonionic detergent treated chicken red blood cells. / Kellermayer, Miklós; Ludány, Andrea; Miseta, A.; Kőszegi, T.; Berta, Gábor; Bogner, P.; Hazlewood, Carlton F.; Cameron, Ivan L.; Wheatley, Denys N.

In: Journal of Cellular Physiology, Vol. 159, No. 2, 05.1994, p. 197-204.

Research output: Contribution to journalArticle

Kellermayer, M, Ludány, A, Miseta, A, Kőszegi, T, Berta, G, Bogner, P, Hazlewood, CF, Cameron, IL & Wheatley, DN 1994, 'Release of potassium, lipids, and proteins from nonionic detergent treated chicken red blood cells', Journal of Cellular Physiology, vol. 159, no. 2, pp. 197-204.
Kellermayer, Miklós ; Ludány, Andrea ; Miseta, A. ; Kőszegi, T. ; Berta, Gábor ; Bogner, P. ; Hazlewood, Carlton F. ; Cameron, Ivan L. ; Wheatley, Denys N. / Release of potassium, lipids, and proteins from nonionic detergent treated chicken red blood cells. In: Journal of Cellular Physiology. 1994 ; Vol. 159, No. 2. pp. 197-204.
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