Release of pancreatic polypeptide in humans by infusion of cholecystokinin

J. Lonovics, S. Guzman, P. Devitt, K. E. Hejtmancik, R. L. Suddith, P. L. Rayford, J. C. Thompson

Research output: Contribution to journalArticle

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Abstract

Plasma levels of pancreatic polypeptide were measured after a test meal and after infusion of graded doses of 99% pure cholecystokinin in 6 healthy volunteers. Initial attempts at sterilization of 99% pure cholecystokinin resulted in complete inactivation. Successful sterilization was accomplished by filtration by using specially treated silver-coated filters. Biologic activity of sterilized material was confirmed with cholecystokinin bioassay, and plasma levels of sterilized cholecystokinin achieved by exogenous infusions were measured with a specific cholecystokinin radioimmunoassay. Significant increases in plasma levels of pancreatic polypeptide were found with a test meal and with infusions of 0.25 and 0.5 μg/kg-hr of 99% pure cholecystokinin. Integrated values of pancreatic polypeptide released by the low-dose and the high-dose infusions of 99% pure cholecystokinin were 59% and 50% of that obtained by food, respectively. Integrated levels of cholecystokinin after 45 min of infusions of 0.25 μg/kg-hr were equal to those after a standard meal. Cholecystokinin, therefore, is an effective humoral releaser of pancreatic polypeptide in humans and may play an important role in the intestinal phase of release of pancreatic polypeptide.

Original languageEnglish
Pages (from-to)817-822
Number of pages6
JournalGastroenterology
Volume79
Issue number5 I
Publication statusPublished - 1980

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Cholecystokinin
Pancreatic Polypeptide
Meals
human PPY protein
Silver
Biological Assay
Radioimmunoassay
Healthy Volunteers
Food

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Lonovics, J., Guzman, S., Devitt, P., Hejtmancik, K. E., Suddith, R. L., Rayford, P. L., & Thompson, J. C. (1980). Release of pancreatic polypeptide in humans by infusion of cholecystokinin. Gastroenterology, 79(5 I), 817-822.

Release of pancreatic polypeptide in humans by infusion of cholecystokinin. / Lonovics, J.; Guzman, S.; Devitt, P.; Hejtmancik, K. E.; Suddith, R. L.; Rayford, P. L.; Thompson, J. C.

In: Gastroenterology, Vol. 79, No. 5 I, 1980, p. 817-822.

Research output: Contribution to journalArticle

Lonovics, J, Guzman, S, Devitt, P, Hejtmancik, KE, Suddith, RL, Rayford, PL & Thompson, JC 1980, 'Release of pancreatic polypeptide in humans by infusion of cholecystokinin', Gastroenterology, vol. 79, no. 5 I, pp. 817-822.
Lonovics J, Guzman S, Devitt P, Hejtmancik KE, Suddith RL, Rayford PL et al. Release of pancreatic polypeptide in humans by infusion of cholecystokinin. Gastroenterology. 1980;79(5 I):817-822.
Lonovics, J. ; Guzman, S. ; Devitt, P. ; Hejtmancik, K. E. ; Suddith, R. L. ; Rayford, P. L. ; Thompson, J. C. / Release of pancreatic polypeptide in humans by infusion of cholecystokinin. In: Gastroenterology. 1980 ; Vol. 79, No. 5 I. pp. 817-822.
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