Release of cytochrome c in MCF-7 cells treated with 7,3′,5′- trihydroxyflavanone of Hydnophytum formicarium

A. Hasmah, L. P. Azimahtol Hawariah, J. Hohmann

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cytochrome c is a central protein that plays a role in triggering apoptosis in cell induced by any agent either drug or plant extracts. Here, we present data of an active antiproliferative agent 7,3′,5′- trihydroxyflavanone (3HFD) that is elucidated from the plant Hydnophytum formicarium showed to trigger cytochrome c release in treated MCF-7 cell. By performing Western Blot, cytochrome c was seen to be elevated throughout the experiments. The increase level of cytochrome c was confirmed by ELISA and remarked as the beginning of the caspase cascade without altering caspase-8 level. In conclusion, the 3HFD seems to significantly induced apoptosis via mitochondria pathway as reflected by up regulation of cytochrome c.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalBiomedical and Pharmacology Journal
Volume2
Issue number1
Publication statusPublished - Jun 2009

Fingerprint

MCF-7 Cells
Cytochromes c
Apoptosis
Caspase 8
Plant Extracts
Caspases
Mitochondria
Up-Regulation
Western Blotting
Enzyme-Linked Immunosorbent Assay
Pharmaceutical Preparations
Proteins

Keywords

  • Cytochrome c
  • Hydnophytum formicarium
  • MCF-7 cels

ASJC Scopus subject areas

  • Pharmacology

Cite this

Release of cytochrome c in MCF-7 cells treated with 7,3′,5′- trihydroxyflavanone of Hydnophytum formicarium. / Hasmah, A.; Azimahtol Hawariah, L. P.; Hohmann, J.

In: Biomedical and Pharmacology Journal, Vol. 2, No. 1, 06.2009, p. 1-6.

Research output: Contribution to journalArticle

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