The release of acetylcholine from human isolated brain cortical slices was studied. Both K excess (47.3 mm) and inhibition of Na+K+-activated ATPase by ouabain (2 × 10-5 m) significantly enhanced the release of acetylcholine. l-Norepinephrine (10-6 m) and phenytoin (10-6 m) reduced the release of acetylcholine evoked by ouabain. When the temperature was decreased to 15°C, ouabain failed to increase the release of acetylcholine, although the resting release was not affected.
ASJC Scopus subject areas
- Developmental Neuroscience