Relaxed chromatin induced by histone deacetylase inhibitors improves the oligonucleotide-directed gene editing in plant cells

Hilda Tiricz, Bettina Nagy, Györgyi Ferenc, Katalin Török, István Nagy, D. Dudits, F. Ayaydin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Improving efficiency of oligonucleotide-directed mutagenesis (ODM) is a prerequisite for wide application of this gene-editing approach in plant science and breeding. Here we have tested histone deacetylase inhibitor treatments for induction of relaxed chromatin and for increasing the efficiency of ODM in cultured maize cells. For phenotypic assay we produced transgenic maize cell lines expressing the non-functional Green Fluorescent Protein (mGFP) gene carrying a TAG stop codon. These transgenic cells were bombarded with corrective oligonucleotide as editing reagent to recover GFP expression. Repair of green fluorescent protein function was monitored by confocal fluorescence microscopy and flow cytometry was used for quantification of correction events. Sequencing PCR fragments of the GFP gene from corrected cells indicated a nucleotide exchange in the stop codon (TAG) from T to G nucleotide that resulted in the restoration of GFP function. We show that pretreatment of maize cells with sodium butyrate (5–10 mM) and nicotinamide (1–5 mM) as known inhibitors of histone deacetylases can cause elevated chromatin sensitivity to DNase I that was visualized in agarose gels and confirmed by the reduced presence of intact PCR template for the inserted exogenous mGFP gene. Maize cells with more relaxed chromatin could serve as an improved recipient for targeted nucleotide exchange as indicated by an average of 2.67- to 3.62-fold increase in GFP-positive cells. Our results stimulate further studies on the role of the condition of the recipient cells in ODM and testing the application of chromatin modifying agents in other, programmable nuclease-based genome-editing techniques in higher plants.

Original languageEnglish
Pages (from-to)179-189
Number of pages11
JournalJournal of Plant Research
Volume131
Issue number1
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

histone deacetylase
oligonucleotides
chromatin
mutagenesis
genes
cells
corn
stop codon
nucleotides
green fluorescent protein
genetically modified organisms
deoxyribonuclease I
nicotinamide
nucleases
fluorescence microscopy
histones
agarose
flow cytometry
pretreatment
cell lines

Keywords

  • Chromatin
  • Gene targeting
  • GFP
  • Maize
  • Oligonucleotide

ASJC Scopus subject areas

  • Plant Science

Cite this

Relaxed chromatin induced by histone deacetylase inhibitors improves the oligonucleotide-directed gene editing in plant cells. / Tiricz, Hilda; Nagy, Bettina; Ferenc, Györgyi; Török, Katalin; Nagy, István; Dudits, D.; Ayaydin, F.

In: Journal of Plant Research, Vol. 131, No. 1, 01.01.2018, p. 179-189.

Research output: Contribution to journalArticle

Tiricz, Hilda ; Nagy, Bettina ; Ferenc, Györgyi ; Török, Katalin ; Nagy, István ; Dudits, D. ; Ayaydin, F. / Relaxed chromatin induced by histone deacetylase inhibitors improves the oligonucleotide-directed gene editing in plant cells. In: Journal of Plant Research. 2018 ; Vol. 131, No. 1. pp. 179-189.
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