In this study, we used genetic-based approaches to estimate population size and structure of Eurasian otter along the Drava River in Hungary, and compared these results to traditional survey-based methods. The relative spraint density of otter was estimated based on the number of fresh (Df) and total number (Dt) of spraints collected on standard routes over a 2-year period. Nine microsatellite loci were screened, generating 17 individual otter genotypes composed of 45 different alleles. The expected heterozygosity ranged from 0.53 to 0.89 and observed heterozygosity from 0.25 to 0.92. The mean density (Dg) estimated over six different sites was 0.17 individuals per km of shoreline. A close correlation was found between the number of genotypes and spraint counts along a standard route (fresh spraints: rP=0.85, P<0.01; total spraints rP=0.76, P<0.05). All genotypes found within the 50 km-long study area were closely related (Dm ranged between 0.08 and 0.21).
- Lutra lutra
- Microsatellite loci
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Animal Science and Zoology