Relationships among porcine and human P[6] rotaviruses

Evidence that the different human P[6] lineages have originated from multiple interspecies transmission events

Vito Martella, K. Bányai, Max Ciarlet, Miren Iturriza-Gómara, Eleonora Lorusso, Simona De Grazia, Serenella Arista, Nicola Decaro, Gabriella Elia, Alessandra Cavalli, Marialaura Corrente, Antonio Lavazza, Rafael Baselga, Canio Buonavoglia

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Porcine rotavirus strains (PoRVs) bearing human-like VP4 P[6] gene alleles were identified. Genetic characterization with either PCR genotyping or sequence analysis allowed to determine the VP7 specificity of the PoRVs as G3, G4, G5 and G9, and the VP6 as genogroup I, that is predictive of a subgroup I specificity. Sequence analysis of the VP8* trypsin-cleavage product of VP4 allowed PoRVs to be characterized further into genetic lineages within the P[6] genotype. Unexpectedly, the strains displayed significantly higher similarity (up to 94.6% and 92.5% at aa and nt level, respectively) to human M37-like P[6] strains (lineage I), serologically classifiable as P2A, or to the atypical Hungarian P[6] human strains (HRVs), designated as lineage V (up to 97.0% aa and 96.1% nt), than to the porcine P[6] strain Gottfried, lineage II (

Original languageEnglish
Pages (from-to)509-519
Number of pages11
JournalVirology
Volume344
Issue number2
DOIs
Publication statusPublished - Jan 20 2006

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Rotavirus
Swine
Sequence Analysis
Genotype
Trypsin
Alleles
Polymerase Chain Reaction
Genes

Keywords

  • Genotyping
  • P[6]
  • Porcine rotaviruses
  • VP4

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Relationships among porcine and human P[6] rotaviruses : Evidence that the different human P[6] lineages have originated from multiple interspecies transmission events. / Martella, Vito; Bányai, K.; Ciarlet, Max; Iturriza-Gómara, Miren; Lorusso, Eleonora; De Grazia, Simona; Arista, Serenella; Decaro, Nicola; Elia, Gabriella; Cavalli, Alessandra; Corrente, Marialaura; Lavazza, Antonio; Baselga, Rafael; Buonavoglia, Canio.

In: Virology, Vol. 344, No. 2, 20.01.2006, p. 509-519.

Research output: Contribution to journalArticle

Martella, V, Bányai, K, Ciarlet, M, Iturriza-Gómara, M, Lorusso, E, De Grazia, S, Arista, S, Decaro, N, Elia, G, Cavalli, A, Corrente, M, Lavazza, A, Baselga, R & Buonavoglia, C 2006, 'Relationships among porcine and human P[6] rotaviruses: Evidence that the different human P[6] lineages have originated from multiple interspecies transmission events', Virology, vol. 344, no. 2, pp. 509-519. https://doi.org/10.1016/j.virol.2005.08.029
Martella, Vito ; Bányai, K. ; Ciarlet, Max ; Iturriza-Gómara, Miren ; Lorusso, Eleonora ; De Grazia, Simona ; Arista, Serenella ; Decaro, Nicola ; Elia, Gabriella ; Cavalli, Alessandra ; Corrente, Marialaura ; Lavazza, Antonio ; Baselga, Rafael ; Buonavoglia, Canio. / Relationships among porcine and human P[6] rotaviruses : Evidence that the different human P[6] lineages have originated from multiple interspecies transmission events. In: Virology. 2006 ; Vol. 344, No. 2. pp. 509-519.
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