Relationship between the occurrence of cysteine in proteins and the complexity of organisms

A. Miseta, Peter Csutora

Research output: Contribution to journalArticle

178 Citations (Scopus)

Abstract

The occurrence and relative positions of cysteine residues were investigated in proteins of various species. Considering random mathematical occurrence for an amino acid coded by two codons (3.28%), cysteine is underrepresented in all organisms investigated. Representation of cysteine appears to correlate positively with the complexity of the organism, ranging between 2.26% in mammals and 0.5% in some members of the Archeabacteria order. This observation, together with the results obtained from comparison of cysteine content of various ribosomal proteins, indicates that evolution takes advantage of increased use of cysteine residues. In all organisms studied except plants, two cysteines are frequently found two amino acid residues apart (C-(X)2-C motif). Such a motif is known to be present in a variety of metal-binding proteins and oxidoreductases. Remarkably, more than 21% of all of cysteines were found within the C-(X)2-C motifs in Archea. This observation may indicate that cysteine appeared in ancient metal-binding proteins first and was introduced into other proteins later.

Original languageEnglish
Pages (from-to)1232-1239
Number of pages8
JournalMolecular Biology and Evolution
Volume17
Issue number8
Publication statusPublished - 2000

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Cysteine
cysteine
protein
organisms
metal binding
Proteins
proteins
amino acid
binding proteins
Carrier Proteins
Metals
metals
Amino Acids
mammal
amino acids
Mammals
organism
Ribosomal Proteins
ribosomal proteins
Archaea

Keywords

  • Amino acids
  • Cysteine
  • Evolution
  • Ribosomal proteins
  • Secondary structure

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Genetics(clinical)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Ecology, Evolution, Behavior and Systematics
  • Agricultural and Biological Sciences (miscellaneous)
  • Molecular Biology

Cite this

Relationship between the occurrence of cysteine in proteins and the complexity of organisms. / Miseta, A.; Csutora, Peter.

In: Molecular Biology and Evolution, Vol. 17, No. 8, 2000, p. 1232-1239.

Research output: Contribution to journalArticle

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