Relation or Raman order parameters to spin labeling parameters.

L. I. Horváth, J. Cirák, L. Vígh

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

For the quantitation of Raman and spin labeling data order parameters are commonly used. The spin label order parameter measured at any depth in the layer is a weighed sum of the segmental order since, due to fast conformational interconversions, each CH2 segment is partly in trans and partly in non-trans, e.g. gauche, kink, jog, etc. conformation during the measurement. The weighing factor, the trans finding probability, varies along the chain (cf. flexibility profile) but its mean value should be equal to the Raman trans order parameter. This correlation is illustrated with the experimental data obtained for dipalmitoyl phosphatidylcholine and n-alcohol mixtures. The rate of rotational diffusion, a dynamical parameter from spin labeling studies, is correlated with the lateral packing density as measured by the Raman lateral order parameter. For the obtained linear correlation a qualitative explanation is given. The effect of a series of long chain alcohols on the phase transition characteristics of dipalmitoyl phosphatidylcholine was investigated. The possible role of hydrogen bonding in the interfacial region is emphasized.

Original languageEnglish
Pages (from-to)237-250
Number of pages14
JournalChemistry and Physics of Lipids
Volume27
Issue number3
DOIs
Publication statusPublished - Oct 1980

Fingerprint

1,2-Dipalmitoylphosphatidylcholine
Labeling
Alcohols
Spin Labels
Phase Transition
Weighing
Hydrogen Bonding
Conformations
Hydrogen bonds
Phase transitions

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Relation or Raman order parameters to spin labeling parameters. / Horváth, L. I.; Cirák, J.; Vígh, L.

In: Chemistry and Physics of Lipids, Vol. 27, No. 3, 10.1980, p. 237-250.

Research output: Contribution to journalArticle

Horváth, L. I. ; Cirák, J. ; Vígh, L. / Relation or Raman order parameters to spin labeling parameters. In: Chemistry and Physics of Lipids. 1980 ; Vol. 27, No. 3. pp. 237-250.
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