Reinforced epithelial barrier integrity via matriptase induction with sphingosine-1-phosphate did not result in disturbances in physiological redox status

E. Pászti-Gere, A. Jerzsele, P. Balla, G. Ujhelyi, A. Székács

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives. The relationship among matriptase function, cellular redox status, and maintenance of intestinal barrier integrity has not been established yet. The aim of this study is to reveal if the crosstalk between matriptase activators and intestinal epithelial monolayers can lead to perturbations in physiological redox regulation in vitro. Methods. The effects of suramin and sphingosine-1-phosphate (S1P) were tested on viability of intestinal porcine epithelial IPEC-J2 cells using MTS assay. Measurements of transepithelial electrical resistance (TER) were performed to determine changes in barrier integrity of cell monolayers. Amplex Red assay was used to monitor extracellular hydrogen peroxide production. Occludin distribution pattern was detected prior to and after matriptase activation using immunofluorescent staining technique. Results. TER reduction was observed in suramin-treated IPEC-J2 cell monolayers, which could be attributed to cell cytotoxic properties of 48 hr 50 M suramin administration. In contrast, S1P treatment increased TER significantly and elevated occludin accumulation in tight junctions. It was also found that extracellular hydrogen peroxide levels were maintained in IPEC-J2 cells exposed to matriptase activators. Discussion. S1P administration not accompanied by redox imbalance might be one of the key strategies in the improvement of barrier function and consequently in the therapy of intestinal inflammations.

Original languageEnglish
Article number9674272
JournalOxidative Medicine and Cellular Longevity
Volume2016
DOIs
Publication statusPublished - 2016

Fingerprint

Suramin
Oxidation-Reduction
Acoustic impedance
Occludin
Monolayers
Electric Impedance
Hydrogen Peroxide
Assays
Crosstalk
Tight Junctions
Chemical activation
Swine
Maintenance
matriptase
sphingosine 1-phosphate
Staining and Labeling
Inflammation
Therapeutics

ASJC Scopus subject areas

  • Cell Biology
  • Ageing
  • Biochemistry

Cite this

Reinforced epithelial barrier integrity via matriptase induction with sphingosine-1-phosphate did not result in disturbances in physiological redox status. / Pászti-Gere, E.; Jerzsele, A.; Balla, P.; Ujhelyi, G.; Székács, A.

In: Oxidative Medicine and Cellular Longevity, Vol. 2016, 9674272, 2016.

Research output: Contribution to journalArticle

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AU - Székács, A.

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