Rehydratable polyacrylamide gels for capillary electrophoresis

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Abstract

A novel separation medium of rehydratable polyacrylamide is described for capillary gel electrophoresis. The dried linear polyacrylamide capable of rehydration to form an aqueous polymeric system which can be filled into a narrow bore capillary column and act as separation matrix. Rehydratable gels provide high resolution and reproducible capillary electrophoresis separations of biopolymers with very long shelf life. This paper relates to separation sieving mediums involving rehydratable gel compositions and methods for using reconstituted linear polyacrylamide solutions as separation mediums in capillary gel electrophoresis of double stranded DNA molecules.

Original languageEnglish
Pages (from-to)1249-1258
Number of pages10
JournalJournal of Liquid Chromatography and Related Technologies
Volume21
Issue number9
DOIs
Publication statusPublished - Jan 1 1998

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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