A novel separation medium of rehydratable polyacrylamide is described for capillary gel electrophoresis. The dried linear polyacrylamide capable of rehydration to form an aqueous polymeric system which can be filled into a narrow bore capillary column and act as separation matrix. Rehydratable gels provide high resolution and reproducible capillary electrophoresis separations of biopolymers with very long shelf life. This paper relates to separation sieving mediums involving rehydratable gel compositions and methods for using reconstituted linear polyacrylamide solutions as separation mediums in capillary gel electrophoresis of double stranded DNA molecules.
|Number of pages||10|
|Journal||Journal of Liquid Chromatography and Related Technologies|
|Publication status||Published - Jan 1 1998|
ASJC Scopus subject areas
- Analytical Chemistry
- Pharmaceutical Science
- Clinical Biochemistry