Regulators of angiogenesis in renal ischemia/reperfusion injury in normotensive and hypertensive rats: Effect of tacrolimus

J. Rajnoch, A. Lodererova, A. Szabo, E. Honsova, A. Vannay, S. Bloudickova, I. Matl, O. Viklicky

Research output: Contribution to journalArticle

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Abstract

The aim of this study was to evaluate the role of proangiogenic growth factors in an experimental model of ischemia/reperfusion injury (I/R) in both normotensive and hypertensive rats. Renal ischemic injury was induced in transgenic rats rendered hypertensive due to renin overproduction [TGR (mREN-2)-27] and in normotensive Hannover Sprague-Dawley rats (HanSD). Animals were treated for 12 weeks with either tacrolimus (TAC, 0.1 mg/kg per day, intramuscularly [IM]) or placebo. After 12 weeks, kidneys were harvested for morphologic, immunohistochemical, and RT-PCR analysis. Both normotensive and hypertensive untreated rats developed significantly greater proteinuria and glomerulosclerosis compared with TAC-treated rats. Immunohistologically, TGR showed higher basic fibroblast growth factor (bFGF) protein expression compared with normotensive HanSD. TAC-treated rats had higher bFGF protein expression than untreated rats. Vascular endothelial growth factor (VEGF) protein expression in glomeruli was more increased in TGR after I/R than in sham-operated animals. TAC-treated TGR hosts developed higher VEGF mRNA expression compared with both untreated and sham groups; however, there were no differences between treated and untreated normotensive HanSD animals. bFGF is involved in the fibrogenesis induced by hypertension and I/R injury. The nature of the increase in proangiogeneic growth factor expression among tacrolimus-treated animals remains to be elucidated.

Original languageEnglish
Pages (from-to)352-354
Number of pages3
JournalTransplantation Proceedings
Volume37
Issue number1
DOIs
Publication statusPublished - Jan 2005

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Tacrolimus
Reperfusion Injury
Kidney
Fibroblast Growth Factor 2
Sprague Dawley Rats
Vascular Endothelial Growth Factor A
Intercellular Signaling Peptides and Proteins
Transgenic Rats
Proteins
Wounds and Injuries
Proteinuria
Renin
Theoretical Models
Placebos
Hypertension
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Surgery
  • Transplantation

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Regulators of angiogenesis in renal ischemia/reperfusion injury in normotensive and hypertensive rats : Effect of tacrolimus. / Rajnoch, J.; Lodererova, A.; Szabo, A.; Honsova, E.; Vannay, A.; Bloudickova, S.; Matl, I.; Viklicky, O.

In: Transplantation Proceedings, Vol. 37, No. 1, 01.2005, p. 352-354.

Research output: Contribution to journalArticle

Rajnoch, J. ; Lodererova, A. ; Szabo, A. ; Honsova, E. ; Vannay, A. ; Bloudickova, S. ; Matl, I. ; Viklicky, O. / Regulators of angiogenesis in renal ischemia/reperfusion injury in normotensive and hypertensive rats : Effect of tacrolimus. In: Transplantation Proceedings. 2005 ; Vol. 37, No. 1. pp. 352-354.
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