Regulation of unbalanced redox homeostasis induced by the expression of wild-type HIV-1 viral protein R (NL4-3Vpr) in fission yeast

Zoltán Gazdag, Timea Stromájer-Rácz, J. Belágyi, Richard Y. Zhao, Robert T. Elder, Eszter Virág, M. Pesti

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The wild-type viral protein R (Vpr) of human immunodeficiency virus type 1 exerts multiple effects on cellular activities during infection, including the induction of cell cycle G2 arrest and the death of human cells and cells of the fission yeast Schizosaccharomyces pombe. In this study, wild-type Vpr (NL4-3Vpr) integrated as a single copy gene in S. pombe chromosome was used to investigate the molecular impact of Vpr on cellular oxidative stress. NL4-3Vpr triggered an atypical response in early (14-h), and a wellregulated oxidative stress response in late (35-h) log-phase cultures. Specifically, NL4-3Vpr expression induced oxidative stress in the 14-h cultures leading, to decreased levels of superoxide anion (O2·-), hydroxyl radical (·OH) and glutathione (GSH), and significantly decreased activities of catalase, glutathione peroxidase, glutathione reductase, glucose-6-phosphate dehydrogenase and glutathione S-transferase. In the 35-h cultures, elevated levels of O2·- and peroxides were accompanied by increased activities of most antioxidant enzymes, suggesting that the Vpr-induced unbalanced redox state of the cells might contribute to the adverse effects in HIV-infected patients.

Original languageEnglish
Pages (from-to)326-338
Number of pages13
JournalActa Biologica Hungarica
Volume66
Issue number3
DOIs
Publication statusPublished - Sep 1 2015

Fingerprint

Human Immunodeficiency Virus vpr Gene Products
vpr Gene Products
Schizosaccharomyces
homeostasis
human immunodeficiency virus
Yeast
Oxidation-Reduction
Oxidative stress
yeast
HIV-1
Homeostasis
Oxidative Stress
protein
Cells
G2 Phase Cell Cycle Checkpoints
Glutathione Reductase
Glucosephosphate Dehydrogenase
Peroxides
Chromosomes
hydroxyl radical

Keywords

  • Fission yeast
  • HIV-1
  • Oxidative stress
  • Schizosaccharomyces pombe
  • Vpr

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology
  • Medicine(all)

Cite this

Regulation of unbalanced redox homeostasis induced by the expression of wild-type HIV-1 viral protein R (NL4-3Vpr) in fission yeast. / Gazdag, Zoltán; Stromájer-Rácz, Timea; Belágyi, J.; Zhao, Richard Y.; Elder, Robert T.; Virág, Eszter; Pesti, M.

In: Acta Biologica Hungarica, Vol. 66, No. 3, 01.09.2015, p. 326-338.

Research output: Contribution to journalArticle

Gazdag, Zoltán ; Stromájer-Rácz, Timea ; Belágyi, J. ; Zhao, Richard Y. ; Elder, Robert T. ; Virág, Eszter ; Pesti, M. / Regulation of unbalanced redox homeostasis induced by the expression of wild-type HIV-1 viral protein R (NL4-3Vpr) in fission yeast. In: Acta Biologica Hungarica. 2015 ; Vol. 66, No. 3. pp. 326-338.
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