Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli

Caroline Blumer, Alexandra Kleefeld, Daniela Lehnen, Margit Heintz, Ulrich Dobrindt, Gábor Nagy, Kai Michaelis, L. Emődy, Tino Polen, Reinhard Rachel, Volker F. Wendisch, Gottfried Unden

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70 Citations (Scopus)

Abstract

Type 1 fimbriae of Escherichia coli facilitate attachment to the host mucosa and promote biofilm formation on abiotic surfaces. The transcriptional regulator LrhA, which is known as a repressor of flagellar, motility and chemotaxis genes, regulates biofilm formation and expression of type 1 fimbriae. Whole-genome expression profiling revealed that inactivation of lrhA results in an increased expression of structural components of type 1 fimbriae. In vitro, LrhA bound to the promoter regions of the two fim recombinases (FimB and FimE) that catalyse the inversion of the fimA promoter, and to the invertible element itself. Translational lacZ fusions with these genes and quantification of fimE transcript levels by real-time PCR showed that LrhA influences type 1 fimbrial phase variation, primarily via activation of FimE, which is required for the ON-to-OFF transition of the fim switch. Enhanced type 1 fimbrial expression as a result of lrhA disruption was confirmed by mannose-sensitive agglutination of yeast cells. Biofilm formation was stimulated by lrhA inactivation and completely suppressed upon LrhA overproduction. The effects of LrhA on biofilm formation were exerted via the changed levels of surface molecules, most probably both flagella and type 1 fimbriae. Together, the data show a role for LrhA as a repressor of type 1 fimbrial expression, and thus as a regulator of the initial stages of biofilm development and, presumably, bacterial adherence to epithelial host cells also.

Original languageEnglish
Pages (from-to)3287-3298
Number of pages12
JournalMicrobiology
Volume151
Issue number10
DOIs
Publication statusPublished - Oct 2005

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Biofilms
Escherichia coli
Recombinases
Flagella
Gene Fusion
Agglutination
Chemotaxis
Mannose
Genetic Promoter Regions
Real-Time Polymerase Chain Reaction
Mucous Membrane
Yeasts
Epithelial Cells
Genome
Genes

ASJC Scopus subject areas

  • Microbiology

Cite this

Blumer, C., Kleefeld, A., Lehnen, D., Heintz, M., Dobrindt, U., Nagy, G., ... Unden, G. (2005). Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli. Microbiology, 151(10), 3287-3298. https://doi.org/10.1099/mic.0.28098-0

Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli. / Blumer, Caroline; Kleefeld, Alexandra; Lehnen, Daniela; Heintz, Margit; Dobrindt, Ulrich; Nagy, Gábor; Michaelis, Kai; Emődy, L.; Polen, Tino; Rachel, Reinhard; Wendisch, Volker F.; Unden, Gottfried.

In: Microbiology, Vol. 151, No. 10, 10.2005, p. 3287-3298.

Research output: Contribution to journalArticle

Blumer, C, Kleefeld, A, Lehnen, D, Heintz, M, Dobrindt, U, Nagy, G, Michaelis, K, Emődy, L, Polen, T, Rachel, R, Wendisch, VF & Unden, G 2005, 'Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli', Microbiology, vol. 151, no. 10, pp. 3287-3298. https://doi.org/10.1099/mic.0.28098-0
Blumer, Caroline ; Kleefeld, Alexandra ; Lehnen, Daniela ; Heintz, Margit ; Dobrindt, Ulrich ; Nagy, Gábor ; Michaelis, Kai ; Emődy, L. ; Polen, Tino ; Rachel, Reinhard ; Wendisch, Volker F. ; Unden, Gottfried. / Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli. In: Microbiology. 2005 ; Vol. 151, No. 10. pp. 3287-3298.
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