Regulation of transcript levels of the Arabidopsis cytochrome P450 genes involved in brassinosteroid biosynthesis

Simona Bancoş, Takahito Nomura, Tatsuro Sato, Gergely Molnár, Gerard J. Bishop, Csaba Koncz, Takao Yokota, Ferenc Nagy, M. Szekeres

Research output: Contribution to journalArticle

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Abstract

Cytochrome P450 enzymes of the closely related CYP90 and CYP85 families catalyze essential oxidative reactions in the biosynthesis of brassinosteroid (BR) hormones. Arabidopsis CYP90B1/DWF4 and CYP90A1/CPD are responsible for respective C-22 and C-23 hydroxylation of the steroid side chain and CYP85A1 catalyzes C-6 oxidation of 6-deoxo intermediates, whereas the functions of CYP90C1/ROT3, CYP90D1, and CYP85A2 are still unknown. Semiquantitative reverse transcriptase-polymerase chain reaction analyses show that transcript levels of CYP85 and CYP90 genes are down-regulated by brassinolide, the end product of the BR biosynthesis pathway. Feedback control of the CYP90C1, CYP9OD1, and CYP85A2 genes by brassinolide suggests that the corresponding enzymes might also participate in BR synthesis. CYP85 and CYP90 mRNAs show strong and transient accumulation during the 1st week of seedling development, as well as characteristic organ-specific distribution. Transcripts of CYP90A1 and CYP85A2 are preferentially represented in shoots and CYP90C1, CYP90D1, and CYP85A1 mRNAs are more abundant in roots, whereas CYP90B1 is ubiquitously expressed. Remarkably, the spatial pattern of CYP90A1 expression is maintained in the BR-insensitive cbb2 mutant, indicating the independence of organ-specific and BR-dependent regulation. Quantitative gas chromatography-mass spectrometry analysis of endogenous BRs in shoots and roots of Arabidopsis, pea (Pisum sativum), and tomato (Lycopersicon esculentum) reveal similar partitioning patterns of BR intermediates in these species. Inverse correlation between CYP90A1/ CPD transcript levels and the amounts of the CYP90A1 substrate 6-deoxocathasterone in shoots and roots suggests that transcriptional regulation plays an important role in controlling BR biosynthesis.

Original languageEnglish
Pages (from-to)504-513
Number of pages10
JournalPlant Physiology
Volume130
Issue number1
DOIs
Publication statusPublished - Sep 2002

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Brassinosteroids
brassinosteroids
Arabidopsis
cytochrome P-450
Cytochrome P-450 Enzyme System
biosynthesis
Genes
genes
brassinolide
Peas
Lycopersicon esculentum
shoots
Messenger RNA
hydroxylation
Hydroxylation
enzymes
Reverse Transcriptase Polymerase Chain Reaction
Seedlings
Solanum lycopersicum var. lycopersicum
Gas Chromatography-Mass Spectrometry

ASJC Scopus subject areas

  • Plant Science

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Regulation of transcript levels of the Arabidopsis cytochrome P450 genes involved in brassinosteroid biosynthesis. / Bancoş, Simona; Nomura, Takahito; Sato, Tatsuro; Molnár, Gergely; Bishop, Gerard J.; Koncz, Csaba; Yokota, Takao; Nagy, Ferenc; Szekeres, M.

In: Plant Physiology, Vol. 130, No. 1, 09.2002, p. 504-513.

Research output: Contribution to journalArticle

Bancoş, S, Nomura, T, Sato, T, Molnár, G, Bishop, GJ, Koncz, C, Yokota, T, Nagy, F & Szekeres, M 2002, 'Regulation of transcript levels of the Arabidopsis cytochrome P450 genes involved in brassinosteroid biosynthesis', Plant Physiology, vol. 130, no. 1, pp. 504-513. https://doi.org/10.1104/pp.005439
Bancoş, Simona ; Nomura, Takahito ; Sato, Tatsuro ; Molnár, Gergely ; Bishop, Gerard J. ; Koncz, Csaba ; Yokota, Takao ; Nagy, Ferenc ; Szekeres, M. / Regulation of transcript levels of the Arabidopsis cytochrome P450 genes involved in brassinosteroid biosynthesis. In: Plant Physiology. 2002 ; Vol. 130, No. 1. pp. 504-513.
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