Regulation of the lateral wall stiffness by acetylcholine and GABA in the outer hair cells of the guinea pig

Tamás József Batta, G. Panyi, Attila Szucs, I. Sziklai

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Acetylcholine (ACh) and GABA, the main neurotransmitters of the efferent innervation of the outer hair cells (OHCs), are assumed to regulate the efficacy of the cochlear amplifier through a variety of mechanisms. The recently described stretch-induced changes of the lateral wall stiffness (regulatory stiffness response) and the stretch-induced slow cell motility of OHCs may be important regulatory mechanisms in this process [J.T. Batta et al. (2003) Eur. J. Physiol. 424, 328-336]. We found that ACh in cochleobasal OHCs significantly reduces the stiffness of the lateral wall but increases the regulatory stiffness response and stretch-induced slow cell motility. Qualitatively similar cellular responses were evoked by GABA in cochleoapical OHCs. The effects of ACh could be inhibited by strychnine, the specific inhibitor of the αg ACh receptors expressed in OHCs, whereas the effects of GABA could be blocked by bicuculline, a specific GABAA receptor antagonist. In the absence of extracellular Ca2+ the effects of ACh and GABA on the regulatory stiffness response were reduced, indicating the involvement of Ca2+ in the control of this process. Based on our results we suggest that efferent innervation protects the organ of Corti against high sound intensities and supports adaptation by modification of the micromechanical properties of OHCs. This could be governed by ACh and GABA indirectly, via the potentiation of stretch-induced cell shortening in a Ca2+-dependent manner, rather than by a direct stiffness regulation-related mechanism.

Original languageEnglish
Pages (from-to)3364-3370
Number of pages7
JournalEuropean Journal of Neuroscience
Volume20
Issue number12
DOIs
Publication statusPublished - Dec 2004

Fingerprint

Outer Auditory Hair Cells
gamma-Aminobutyric Acid
Acetylcholine
Guinea Pigs
Cell Movement
GABA-A Receptor Antagonists
Organ of Corti
Strychnine
Bicuculline
Cochlea
Cholinergic Receptors
Neurotransmitter Agents

Keywords

  • Acetylcholine
  • Ca
  • GABA
  • Slow cell motility
  • Stiffness parameter

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Regulation of the lateral wall stiffness by acetylcholine and GABA in the outer hair cells of the guinea pig. / Batta, Tamás József; Panyi, G.; Szucs, Attila; Sziklai, I.

In: European Journal of Neuroscience, Vol. 20, No. 12, 12.2004, p. 3364-3370.

Research output: Contribution to journalArticle

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