Regulation of the Equilibrium between Closed and Open Conformations of Annexin A2 by N-Terminal Phosphorylation and S100A4-Binding

Péter Ecsédi, Bence Kiss, Gergő Gógl, László Radnai, L. Buday, Kitti Koprivanacz, K. Liliom, Ibolya Leveles, B. Vértessy, Norbert Jeszenői, C. Hetényi, G. Schlosser, Gergely Katona, L. Nyitray

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Annexin A2 (ANXA2) has a versatile role in membrane-associated functions including membrane aggregation, endo- and exocytosis, and it is regulated by post-translational modifications and protein-protein interactions through the unstructured N-terminal domain (NTD). Our sequence analysis revealed a short motif responsible for clamping the NTD to the C-terminal core domain (CTD). Structural studies indicated that the flexibility of the NTD and CTD are interrelated and oppositely regulated by Tyr24 phosphorylation and Ser26Glu phosphomimicking mutation. The crystal structure of the ANXA2-S100A4 complex showed that asymmetric binding of S100A4 induces dislocation of the NTD from the CTD and, similar to the Ser26Glu mutation, unmasks the concave side of ANXA2. In contrast, pTyr24 anchors the NTD to the CTD and hampers the membrane-bridging function. This inhibition can be restored by S100A4 and S100A10 binding. Based on our results we provide a structural model for regulation of ANXA2-mediated membrane aggregation by NTD phosphorylation and S100 binding.

Original languageEnglish
Pages (from-to)1195-1207.e5
JournalStructure
Volume25
Issue number8
DOIs
Publication statusPublished - Aug 1 2017

Fingerprint

Annexins
Annexin A2
Phosphorylation
Membranes
Mutation
Structural Models
Exocytosis
Post Translational Protein Processing
Endocytosis
Constriction
Sequence Analysis
Proteins

Keywords

  • asymmetric interaction
  • Ca binding
  • conformational fluctuation
  • molecular dynamics simulation
  • protein phosphorylation
  • X-ray crystallography

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Regulation of the Equilibrium between Closed and Open Conformations of Annexin A2 by N-Terminal Phosphorylation and S100A4-Binding. / Ecsédi, Péter; Kiss, Bence; Gógl, Gergő; Radnai, László; Buday, L.; Koprivanacz, Kitti; Liliom, K.; Leveles, Ibolya; Vértessy, B.; Jeszenői, Norbert; Hetényi, C.; Schlosser, G.; Katona, Gergely; Nyitray, L.

In: Structure, Vol. 25, No. 8, 01.08.2017, p. 1195-1207.e5.

Research output: Contribution to journalArticle

Ecsédi, Péter ; Kiss, Bence ; Gógl, Gergő ; Radnai, László ; Buday, L. ; Koprivanacz, Kitti ; Liliom, K. ; Leveles, Ibolya ; Vértessy, B. ; Jeszenői, Norbert ; Hetényi, C. ; Schlosser, G. ; Katona, Gergely ; Nyitray, L. / Regulation of the Equilibrium between Closed and Open Conformations of Annexin A2 by N-Terminal Phosphorylation and S100A4-Binding. In: Structure. 2017 ; Vol. 25, No. 8. pp. 1195-1207.e5.
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