Regulation of stress-induced transcriptional changes in the hypothalamic neurosecretory neurons

K. Kovács, Paul E. Sawchenko

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Transcriptional changes in corticotropin-releasing factor (CRF) and arginine vasopressin (AVP) gene expression were studied by in situ hybridization histochemistry using cRNA probes directed against intronic sequences. Acute ether stress resulted in a rapid induction of CRF and a delayed activation of vasopressin heteronuclear (hn)RNA in the parvocellular neurosecretory neurons within the paraventricular nucleus (PVN) of the hypothalamus. To explore possible molecular mechanisms regulating stress-related neuropeptide expression in vivo, the time-courses of stress-induced activation of different transcription factor classes were compared to that of changes in neuropeptide transcription. The peak of CRF transcription was parallel to that of cAMP response-element binding protein (CREB) phosphorylation but preceded the induction of c-fos and NGFI-B mRNAs and Fos protein. In contrast, AVP expression occurred in step with immediate-early gene (IEG) responses, suggesting involvement of different mechanisms underlying stress-induced neuropeptide responses. The interference of glucocorticoid hormones with stress-induced neuropeptide and transcription-factor responses has also been revealed in rats acutely or chronically pretreated with glucocorticoids. Acute dexamethasone injection did not prevent neuropeptide and transcription factor responses to ether inhalation, whereas chronic corticosterone administration completely blocked IEG and neuropeptide induction in the stress-related neurosecretory neurons.

Original languageEnglish
Pages (from-to)125-133
Number of pages9
JournalJournal of Molecular Neuroscience
Volume7
Issue number2
DOIs
Publication statusPublished - Jun 1996

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Neuropeptides
Neurons
Corticotropin-Releasing Hormone
Immediate-Early Genes
Transcription Factors
Arginine Vasopressin
Transcription
Ether
Glucocorticoids
Genes
Chemical activation
Proto-Oncogene Proteins c-fos
Cyclic AMP Response Element-Binding Protein
Complementary RNA
Phosphorylation
Paraventricular Hypothalamic Nucleus
Corticosterone
Vasopressins
Gene expression
Inhalation

Keywords

  • corticotropin-releasing factor
  • CREB
  • Stress
  • transcription factors
  • vasopressin

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Biochemistry
  • Genetics
  • Neuroscience(all)

Cite this

Regulation of stress-induced transcriptional changes in the hypothalamic neurosecretory neurons. / Kovács, K.; Sawchenko, Paul E.

In: Journal of Molecular Neuroscience, Vol. 7, No. 2, 06.1996, p. 125-133.

Research output: Contribution to journalArticle

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