Regulation of pituitary inhibin/activin subunits and follistatin gene expression by GnRH in female rats

Petra Popovics, Z. Rékási, Alan J. Stewart, Magdolna Kovacs

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Pituitary inhibin B, activin B, and follistatin are local regulators of FSH. Activin B is a homodimeric molecule (βB-βB), while inhibin B contains an a and a βB subunit. The regulation of gene expression of α, βB, and follistatin by local and endocrine hormones was examined in pituitaries from female rats and in perifused pituitary cells by RT-PCR. Ovariectomy (OVX) induced an elevation in the mRNA level of α and βB subunits and follistatin. Short-term (4 h) treatment of pituitary cells with GnRH decreased both the inhibin α and the inhibin/activin βB subunit mRNA levels, while long-term treatment (20 h) with 100 nM GnRH stimulated the expression of both subunits. In contrast, the mRNA level of follistatin was elevated after the short-term GnRH treatment. Long-term exposure of pituitary cells to estradiol and inhibin B suppressed the mRNA expression of βB and had no effect on the expression of α subunit and follistatin. Our results demonstrate that the increased expressions of inhibin/activin subunits and follistatin in the post-OVX period can be induced by the lack of gonadal negative feedback, resulting in a high GnRH environment in the pituitary. This study reports for the first time that GnRH administered in high doses and for a long period stimulates the gene expression of inhibin/activin subunits and thereby may contribute to the stimulatory effect of OVX on the expression of these genes.

Original languageEnglish
Pages (from-to)71-79
Number of pages9
JournalJournal of Endocrinology
Volume210
Issue number1
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Follistatin
Activins
Inhibins
Gonadotropin-Releasing Hormone
Gene Expression
Messenger RNA
Gene Expression Regulation
Ovariectomy
Estradiol
Hormones
Polymerase Chain Reaction
activin B
inhibin B

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Regulation of pituitary inhibin/activin subunits and follistatin gene expression by GnRH in female rats. / Popovics, Petra; Rékási, Z.; Stewart, Alan J.; Kovacs, Magdolna.

In: Journal of Endocrinology, Vol. 210, No. 1, 07.2011, p. 71-79.

Research output: Contribution to journalArticle

Popovics, Petra ; Rékási, Z. ; Stewart, Alan J. ; Kovacs, Magdolna. / Regulation of pituitary inhibin/activin subunits and follistatin gene expression by GnRH in female rats. In: Journal of Endocrinology. 2011 ; Vol. 210, No. 1. pp. 71-79.
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