Regulation of oxidative stress-induced cytotoxic processes of citrinin in the fission yeast Schizosaccharomyces pombe

Gábor Máté, Zoltán Gazdag, Nóra Mike, Gábor Papp, I. Pócsi, M. Pesti

Research output: Contribution to journalArticle

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Abstract

In this study, the citrinin (CTN)-induced accumulation of reactive oxygen species (ROS) and the regulation of the activities of antioxidant enzymes were investigated in acute toxicity tests in Schizosaccharomyces pombe. 30% of the CTN was accumulated by the cells in 1000 μM CTN solution. In comparison with the control, exposure of 107 cells ml-1 to 1000 μM CTN for 60 min at pH = 4.5 induced significantly (p <1%) elevated levels of peroxides and total ROS, but not of superoxide or hydroxyl radicals, while there was a 3-fold increase in the concentration of glutathione. ROS-induced adaptation processes at cell and molecular levels via activation of the redox-sensitive transcription factors Pap1 and (in part) Atf1 resulted in significantly increased specific activities of glutathione peroxidases, glucose-6-phosphate dehydrogenase and glutathione S-transferase and in decreased levels of catalase and glutathione reductase, but no changes were detected in the activities of superoxide dismutases. This treatment caused a G2/M cell cycle arrest and elevated the number of fragmented nuclei, which is one of the markers of apoptosis. Comparison of these results with those for the positive control, 200 μM H2O2, suggested that CTN induced a medium level of oxidative stress.

Original languageEnglish
Pages (from-to)155-166
Number of pages12
JournalToxicon
Volume90
DOIs
Publication statusPublished - 2014

Fingerprint

Citrinin
Oxidative stress
Schizosaccharomyces
Yeast
Oxidative Stress
Reactive Oxygen Species
Exposure controls
Acute Toxicity Tests
G2 Phase Cell Cycle Checkpoints
Glutathione Reductase
Glucosephosphate Dehydrogenase
Peroxides
Glutathione Peroxidase
Glutathione Transferase
Superoxides
Hydroxyl Radical
Catalase
Superoxide Dismutase
Oxidation-Reduction
Glutathione

Keywords

  • Antioxidant enzyme
  • Citrinin
  • Fission yeast
  • Glutathione
  • Reactive oxygen species

ASJC Scopus subject areas

  • Toxicology
  • Medicine(all)

Cite this

Regulation of oxidative stress-induced cytotoxic processes of citrinin in the fission yeast Schizosaccharomyces pombe. / Máté, Gábor; Gazdag, Zoltán; Mike, Nóra; Papp, Gábor; Pócsi, I.; Pesti, M.

In: Toxicon, Vol. 90, 2014, p. 155-166.

Research output: Contribution to journalArticle

Máté, Gábor ; Gazdag, Zoltán ; Mike, Nóra ; Papp, Gábor ; Pócsi, I. ; Pesti, M. / Regulation of oxidative stress-induced cytotoxic processes of citrinin in the fission yeast Schizosaccharomyces pombe. In: Toxicon. 2014 ; Vol. 90. pp. 155-166.
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