Regulation of histidine decarboxylase activity in rat hypothalamus in vitro by ATP and cyclic AMP: Enzyme inactivation under phosphorylating conditions

Zsuzsanna Huszti, K. Magyar

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10 Citations (Scopus)

Abstract

In vitro, hypothalamic HD1 from rat, could strikingly be inhibited by ATP and cyclic AMP. The enzyme inhibition is partially dependent upon Mg2+ and the circumstances favourable for a cAMP-dependent phosphorylation. An almost complete inhibition could be achieved by incubating the homogenate of the hypothalamus under phosphorylating conditions (ATP, cAMP, Mg2+ and IBMX) in the presence of a cAMP-dependent protein kinase (obtained from bovine thymus). Cyclic nucleotides and ATP alone elicit only moderate inhibitions on the hypothalamic HD activity. Neither ATP, nor cAMP, added alone or in combinations, alter the total brain or the hypothalamic HNMT from guinea-pigs or rats in concentrations up to 10-3M. Results suggest that hypothalamic HD is regulated through a cAMP-dependent process, probable a direct phosphorylation, via a cAMP-dependent protein kinase.

Original languageEnglish
Pages (from-to)546-549
Number of pages4
JournalAgents and Actions
Volume14
Issue number3-4
DOIs
Publication statusPublished - May 1984

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Histidine Decarboxylase
Cyclic AMP
Hypothalamus
Rats
Adenosine Triphosphate
Phosphorylation
Enzymes
Cyclic AMP-Dependent Protein Kinases
Enzyme inhibition
1-Methyl-3-isobutylxanthine
Thymus
Cyclic Nucleotides
Thymus Gland
Brain
Guinea Pigs
In Vitro Techniques

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)

Cite this

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abstract = "In vitro, hypothalamic HD1 from rat, could strikingly be inhibited by ATP and cyclic AMP. The enzyme inhibition is partially dependent upon Mg2+ and the circumstances favourable for a cAMP-dependent phosphorylation. An almost complete inhibition could be achieved by incubating the homogenate of the hypothalamus under phosphorylating conditions (ATP, cAMP, Mg2+ and IBMX) in the presence of a cAMP-dependent protein kinase (obtained from bovine thymus). Cyclic nucleotides and ATP alone elicit only moderate inhibitions on the hypothalamic HD activity. Neither ATP, nor cAMP, added alone or in combinations, alter the total brain or the hypothalamic HNMT from guinea-pigs or rats in concentrations up to 10-3M. Results suggest that hypothalamic HD is regulated through a cAMP-dependent process, probable a direct phosphorylation, via a cAMP-dependent protein kinase.",
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