Regulation of free amino acid and polyamine levels during cold acclimation in wheat

G. Kocsy, L. Simon-Sarkadi, Zita Kovács, Ákos Boldizsár, Csongor Sovány, Klára Kirsch, G. Galiba

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effect of cold acclimation on free amino acids and polyamines was compared at metabolite and transcript level in wheat chromosome 5A substitution lines with different freezing tolerance. Three weeks at 2°C resulted in increased H 2O 2 content which alteration may affect the metabolism of these compounds. The concentration of most free amino acids gradually increased during the treatment. The expression of the genes encoding enzymes of amino acid metabolism, thus that of pyrroline-5-carboxylate reductase, glutamate synthetase and aspartate transferase had a fast transient increase during the first days of growth at 2°C. The concentration of the polyamines putrescine and spermidine exhibited a great increase in all three genotypes, while spermine and cadaverine levels showed only slight changes. Among the genes related to the polyamine metabolism, the transcript level of those ones encoding arginine and ornithine decarboxylase increased and that of the spermidine synthetase did not change. The observed changes in metabolite and transcript levels were controlled by chromo some 5A in the case of Pro, Glu and putrescine. The present result show that free amino acids and polyamines play an important role in the cold acclimation and their levels are regulated at the transcriptional level.

Original languageEnglish
Pages (from-to)91-93
Number of pages3
JournalActa Biologica Szegediensis
Volume55
Issue number1
Publication statusPublished - 2011

Fingerprint

Acclimatization
Polyamines
polyamines
free amino acids
Triticum
acclimation
Metabolism
Amino Acids
wheat
Putrescine
putrescine
Metabolites
Pyrroline Carboxylate Reductases
Spermidine Synthase
Cadaverine
metabolites
arginine decarboxylase
substitution lines
ornithine decarboxylase
Gene encoding

Keywords

  • Cold acclimation
  • Free amino acid
  • Polyamine
  • Wheaty

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Regulation of free amino acid and polyamine levels during cold acclimation in wheat. / Kocsy, G.; Simon-Sarkadi, L.; Kovács, Zita; Boldizsár, Ákos; Sovány, Csongor; Kirsch, Klára; Galiba, G.

In: Acta Biologica Szegediensis, Vol. 55, No. 1, 2011, p. 91-93.

Research output: Contribution to journalArticle

Kocsy, G. ; Simon-Sarkadi, L. ; Kovács, Zita ; Boldizsár, Ákos ; Sovány, Csongor ; Kirsch, Klára ; Galiba, G. / Regulation of free amino acid and polyamine levels during cold acclimation in wheat. In: Acta Biologica Szegediensis. 2011 ; Vol. 55, No. 1. pp. 91-93.
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