Regulation of formation of the intracellular β-galactosidase activity of Aspergillus nidulans

E. Fekete, L. Karaffa, E. Sándor, Bernhard Seiboth, Sándor Biró, A. Szentirmai, Christian P. Kubicek

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The regulation of formation of the single intra-cellular β-galactosidase activity of Aspergillus nidulans was investigated. β-Galactosidase was not formed during growth on glucose or glycerol, but was rapidly induced during growth on lactose or D-galactose. L-Arabinose, and - with lower efficacy - D-xylose also induced β-galactosidase activity. Addition of glucose to cultures growing on lactose led to a rapid decrease in β-galactosidase activity. In contrast, in cultures growing on D-galactose, addition of glucose decreased the activity of β-galactosidase only slightly. Glucose inhibited the uptake of lactose, but not of D-galactose, and required the carbon catabolite re-pressor CreA for this. In addition, CreA also repressed the formation of basal levels of β-galactosidase and partially interfered with the induction of β-galactosidase by D-galactose, L-arabinose, and D-xylose. D-Galactose phosphorylation was not necessary for β-galactosidase induction, since induction by D-galactose occurred in an A. nidulans mutant defective in galactose kinase, and by the non-metabolizable D-galactose analogue fucose in the wild-type strain. Interestingly, a mutant in galactose-1-phosphate uridylyl transferase produced β-galactosidase at a low, constitutive level even on glucose and glycerol and was no longer inducible by D-galactose, whereas it was still inducible by L-arabinose. We conclude that biosynthesis of the intracellular β-galactosidase of A. nidulans is regulated by CreA, partially repressed by galactose-1-phosphate uridylyl transferase, and induced by D-galactose and L-arabinose in independent ways.

Original languageEnglish
Pages (from-to)7-14
Number of pages8
JournalArchives of Microbiology
Volume179
Issue number1
DOIs
Publication statusPublished - 2003

Fingerprint

Galactosidases
Aspergillus nidulans
Aspergillus
Galactose
Arabinose
Glucose
Lactose
Xylose
Transferases
Glycerol
Phosphorylation
Fucose
Biosynthesis
Growth
Phosphotransferases
Carbon

Keywords

  • β-Galactosidase
  • Arabinose
  • Aspergillus nidulans
  • CreA
  • Galactose
  • Lactose

ASJC Scopus subject areas

  • Microbiology

Cite this

Regulation of formation of the intracellular β-galactosidase activity of Aspergillus nidulans. / Fekete, E.; Karaffa, L.; Sándor, E.; Seiboth, Bernhard; Biró, Sándor; Szentirmai, A.; Kubicek, Christian P.

In: Archives of Microbiology, Vol. 179, No. 1, 2003, p. 7-14.

Research output: Contribution to journalArticle

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