Regulation of cytotoxic, non-estrogenic, oxidative stress-induced processes of zearalenone in the fission yeast Schizosaccharomyces pombe

Nóra Mike, Gábor Papp, Milan Čertik, Zsuzsanna Czibulya, S. Kunsági-Máté, I. Ember, C. Vágvölgyi, M. Pesti, Zoltán Gazdag

Research output: Contribution to journalArticle

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Abstract

This study investigates the non-estrogenic mode of zearalenone (ZEA) toxicity in a novel aspect via accumulation of reactive oxygen species (ROS) and the regulation of the activities of antioxidant enzymes in the Schizosaccharomyces pombe in acute toxicity tests. In comparison with the control, 500μM ZEA treatment caused 66% decrease in the concentration of glutathione (GSH), which was a consequence, in the absence of ZEA-GSH interaction, of the GSH-consuming processes of the antioxidant system; this depletion of GSH initiated a 1.8- and 2.0-fold accumulation of the superoxide anion and hydrogen peroxide, but did not increase the concentration of the hydroxyl radical; ROS-induced adaptation processes via activation of the Pap1 transcription factor resulted in significantly increased activities of superoxide dismutases, catalase, glutathione reductase and glutathione S-transferase, and decreased activities of glutathione peroxidase and glucose-6-phosphate dehydrogenase. This treatment altered the sterol composition of the cells by inducing decreased concentrations of ergosterol, squalene and 24-methylene-24,25-hydrolanosterol, and also elevated the number of fragmented nuclei. Cells strived to correct the unbalanced redox state by regulation of the antioxidant system, but this was not enough to defend the cells from the disturbed sterol composition, the cell cycle arrest, and the fragmentation of nuclei.

Original languageEnglish
Pages (from-to)130-143
Number of pages14
JournalToxicon
Volume73
DOIs
Publication statusPublished - 2013

Fingerprint

Zearalenone
Oxidative stress
Schizosaccharomyces
Yeast
Oxidative Stress
Antioxidants
Sterols
Toxicity
Reactive Oxygen Species
Acute Toxicity Tests
Squalene
Ergosterol
Glutathione Reductase
Glucosephosphate Dehydrogenase
Glutathione Peroxidase
Cell Cycle Checkpoints
Glutathione Transferase
Chemical analysis
Superoxides
Hydroxyl Radical

Keywords

  • Ergosterol
  • Glutathione
  • Oxidative stress
  • Regulation
  • Schizosaccharomyces pombe
  • Zearalenone

ASJC Scopus subject areas

  • Toxicology
  • Medicine(all)

Cite this

Regulation of cytotoxic, non-estrogenic, oxidative stress-induced processes of zearalenone in the fission yeast Schizosaccharomyces pombe. / Mike, Nóra; Papp, Gábor; Čertik, Milan; Czibulya, Zsuzsanna; Kunsági-Máté, S.; Ember, I.; Vágvölgyi, C.; Pesti, M.; Gazdag, Zoltán.

In: Toxicon, Vol. 73, 2013, p. 130-143.

Research output: Contribution to journalArticle

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